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G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis

BACKGROUND: Forkhead box protein 3 (FoxP3) is an important factor for development and function of Regulatory T cells (Treg). Studies have found an association between common gene polymorphisms in FoxP3 and some infectious diseases. The aim of this study was to evaluate possible associations between...

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Autores principales: Beiranvand, Elham, Abediankenari, Saeid, Khani, Soghra, Hosseini, Hamideh Mahmoodzadeh, Zeinali, Sirous, Beiranvand, Behnoush, Goudarzi, Mehdi, Seyedjavadi, Sima Sadat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637085/
https://www.ncbi.nlm.nih.gov/pubmed/29020928
http://dx.doi.org/10.1186/s12879-017-2762-5
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author Beiranvand, Elham
Abediankenari, Saeid
Khani, Soghra
Hosseini, Hamideh Mahmoodzadeh
Zeinali, Sirous
Beiranvand, Behnoush
Goudarzi, Mehdi
Seyedjavadi, Sima Sadat
author_facet Beiranvand, Elham
Abediankenari, Saeid
Khani, Soghra
Hosseini, Hamideh Mahmoodzadeh
Zeinali, Sirous
Beiranvand, Behnoush
Goudarzi, Mehdi
Seyedjavadi, Sima Sadat
author_sort Beiranvand, Elham
collection PubMed
description BACKGROUND: Forkhead box protein 3 (FoxP3) is an important factor for development and function of Regulatory T cells (Treg). Studies have found an association between common gene polymorphisms in FoxP3 and some infectious diseases. The aim of this study was to evaluate possible associations between two Single nucleotide polymorphisms (SNPs) in the promoter of the FoxP3 gene to susceptibility to tuberculosis (TB) and the alteration of Foxp3 gene expression. METHODS: The pattern distribution of genotype at two position, −3279 A > C (rs3761548) and −924 A > G (rs2232365) on the promoter of FoxP3 gene was evaluated using polymerase chain reaction-single specific primer (PCR-SSP) method in 183 tuberculosis patients and 183 healthy control. In addition the quantity of FoxP3 gene expression at mRNA level was identified by the real-time PCR. RESULTS: The frequency of G allele at −924 A > G was significantly higher was higher in TB patients (59.5%) than control group (39.5%) (P ≤ 0.05). In addition, our data viewed approximately 5- folds more FoxP3 gene expression in female patients with GG genotype in comparison to female healthy cases with the same genotype (P ≤ 0.001). There was no statistically significant differences between the distribution pattern of −3279 A > C polymorphism in patients and healthy individuals along with it effect on the FoxP3 gene expression among both groups (P > 0.05). CONCLUSIONS: Our outcome suggests that the −924 A > G polymorphism leads to enhance FoxP3 gene expression and susceptibility to tuberculosis in the sex dependent manner. This event may rise the count of Treg cells and modulate the immune response against tuberculosis.
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spelling pubmed-56370852017-10-18 G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis Beiranvand, Elham Abediankenari, Saeid Khani, Soghra Hosseini, Hamideh Mahmoodzadeh Zeinali, Sirous Beiranvand, Behnoush Goudarzi, Mehdi Seyedjavadi, Sima Sadat BMC Infect Dis Research Article BACKGROUND: Forkhead box protein 3 (FoxP3) is an important factor for development and function of Regulatory T cells (Treg). Studies have found an association between common gene polymorphisms in FoxP3 and some infectious diseases. The aim of this study was to evaluate possible associations between two Single nucleotide polymorphisms (SNPs) in the promoter of the FoxP3 gene to susceptibility to tuberculosis (TB) and the alteration of Foxp3 gene expression. METHODS: The pattern distribution of genotype at two position, −3279 A > C (rs3761548) and −924 A > G (rs2232365) on the promoter of FoxP3 gene was evaluated using polymerase chain reaction-single specific primer (PCR-SSP) method in 183 tuberculosis patients and 183 healthy control. In addition the quantity of FoxP3 gene expression at mRNA level was identified by the real-time PCR. RESULTS: The frequency of G allele at −924 A > G was significantly higher was higher in TB patients (59.5%) than control group (39.5%) (P ≤ 0.05). In addition, our data viewed approximately 5- folds more FoxP3 gene expression in female patients with GG genotype in comparison to female healthy cases with the same genotype (P ≤ 0.001). There was no statistically significant differences between the distribution pattern of −3279 A > C polymorphism in patients and healthy individuals along with it effect on the FoxP3 gene expression among both groups (P > 0.05). CONCLUSIONS: Our outcome suggests that the −924 A > G polymorphism leads to enhance FoxP3 gene expression and susceptibility to tuberculosis in the sex dependent manner. This event may rise the count of Treg cells and modulate the immune response against tuberculosis. BioMed Central 2017-10-11 /pmc/articles/PMC5637085/ /pubmed/29020928 http://dx.doi.org/10.1186/s12879-017-2762-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Beiranvand, Elham
Abediankenari, Saeid
Khani, Soghra
Hosseini, Hamideh Mahmoodzadeh
Zeinali, Sirous
Beiranvand, Behnoush
Goudarzi, Mehdi
Seyedjavadi, Sima Sadat
G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title_full G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title_fullStr G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title_full_unstemmed G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title_short G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
title_sort g allele at −924 a > g position of foxp3 gene promoter as a risk factor for tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637085/
https://www.ncbi.nlm.nih.gov/pubmed/29020928
http://dx.doi.org/10.1186/s12879-017-2762-5
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