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Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus

BACKGROUND: The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Compared to other mosquito species, Ae. albopictus females exhibit a generalis...

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Autores principales: Lombardo, Fabrizio, Salvemini, Marco, Fiorillo, Carmine, Nolan, Tony, Zwiebel, Laurence J., Ribeiro, José M., Arcà, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637092/
https://www.ncbi.nlm.nih.gov/pubmed/29020917
http://dx.doi.org/10.1186/s12864-017-4144-1
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author Lombardo, Fabrizio
Salvemini, Marco
Fiorillo, Carmine
Nolan, Tony
Zwiebel, Laurence J.
Ribeiro, José M.
Arcà, Bruno
author_facet Lombardo, Fabrizio
Salvemini, Marco
Fiorillo, Carmine
Nolan, Tony
Zwiebel, Laurence J.
Ribeiro, José M.
Arcà, Bruno
author_sort Lombardo, Fabrizio
collection PubMed
description BACKGROUND: The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Compared to other mosquito species, Ae. albopictus females exhibit a generalist host seeking as well as a very aggressive biting behaviour that are responsible for its high degree of nuisance. Several complex mosquito behaviours such as host seeking, feeding, mating or oviposition rely on olfactory stimuli that target a range of sensory neurons localized mainly on specialized head appendages such as antennae, maxillary palps and the mouthparts. RESULTS: With the aim to describe the Ae. albopictus olfactory repertoire we have used RNA-seq to reveal the transcriptome profiles of female antennae and maxillary palps. Male heads and whole female bodies were employed as reference for differential expression analysis. The relative transcript abundance within each tissue (TPM, transcripts per kilobase per million) and the pairwise differential abundance in the different tissues (fold change values and false discovery rates) were evaluated. Contigs upregulated in the antennae (620) and maxillary palps (268) were identified and relative GO and PFAM enrichment profiles analysed. Chemosensory genes were described: overall, 77 odorant binding proteins (OBP), 82 odorant receptors (OR), 60 ionotropic receptors (IR) and 30 gustatory receptors (GR) were identified by comparative genomics and transcriptomics. In addition, orthologs of genes expressed in the female/male maxillary palps and/or antennae and involved in thermosensation (e.g. pyrexia and arrestin1), mechanosensation (e.g. piezo and painless) and neuromodulation were classified. CONCLUSIONS: We provide here the first detailed transcriptome of the main Ae. albopictus sensory appendages, i.e. antennae and maxillary palps. A deeper knowledge of the olfactory repertoire of the tiger mosquito will help to better understand its biology and may pave the way to design new attractants/repellents. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4144-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-56370922017-10-18 Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus Lombardo, Fabrizio Salvemini, Marco Fiorillo, Carmine Nolan, Tony Zwiebel, Laurence J. Ribeiro, José M. Arcà, Bruno BMC Genomics Research Article BACKGROUND: The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Compared to other mosquito species, Ae. albopictus females exhibit a generalist host seeking as well as a very aggressive biting behaviour that are responsible for its high degree of nuisance. Several complex mosquito behaviours such as host seeking, feeding, mating or oviposition rely on olfactory stimuli that target a range of sensory neurons localized mainly on specialized head appendages such as antennae, maxillary palps and the mouthparts. RESULTS: With the aim to describe the Ae. albopictus olfactory repertoire we have used RNA-seq to reveal the transcriptome profiles of female antennae and maxillary palps. Male heads and whole female bodies were employed as reference for differential expression analysis. The relative transcript abundance within each tissue (TPM, transcripts per kilobase per million) and the pairwise differential abundance in the different tissues (fold change values and false discovery rates) were evaluated. Contigs upregulated in the antennae (620) and maxillary palps (268) were identified and relative GO and PFAM enrichment profiles analysed. Chemosensory genes were described: overall, 77 odorant binding proteins (OBP), 82 odorant receptors (OR), 60 ionotropic receptors (IR) and 30 gustatory receptors (GR) were identified by comparative genomics and transcriptomics. In addition, orthologs of genes expressed in the female/male maxillary palps and/or antennae and involved in thermosensation (e.g. pyrexia and arrestin1), mechanosensation (e.g. piezo and painless) and neuromodulation were classified. CONCLUSIONS: We provide here the first detailed transcriptome of the main Ae. albopictus sensory appendages, i.e. antennae and maxillary palps. A deeper knowledge of the olfactory repertoire of the tiger mosquito will help to better understand its biology and may pave the way to design new attractants/repellents. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4144-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-11 /pmc/articles/PMC5637092/ /pubmed/29020917 http://dx.doi.org/10.1186/s12864-017-4144-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lombardo, Fabrizio
Salvemini, Marco
Fiorillo, Carmine
Nolan, Tony
Zwiebel, Laurence J.
Ribeiro, José M.
Arcà, Bruno
Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title_full Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title_fullStr Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title_full_unstemmed Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title_short Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
title_sort deciphering the olfactory repertoire of the tiger mosquito aedes albopictus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637092/
https://www.ncbi.nlm.nih.gov/pubmed/29020917
http://dx.doi.org/10.1186/s12864-017-4144-1
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