Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases

High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells. Single guide RNAs harboring precise, perfectly matched 20-nu...

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Detalles Bibliográficos
Autores principales: Zhang, Dingbo, Zhang, Huawei, Li, Tingdong, Chen, Kunling, Qiu, Jin-Long, Gao, Caixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637269/
https://www.ncbi.nlm.nih.gov/pubmed/29020979
http://dx.doi.org/10.1186/s13059-017-1325-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637269/
https://www.ncbi.nlm.nih.gov/pubmed/29020979
http://dx.doi.org/10.1186/s13059-017-1325-9

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