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Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST ha...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637851/ https://www.ncbi.nlm.nih.gov/pubmed/29104724 http://dx.doi.org/10.1155/2017/1956104 |
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author | Harada, Fumiya Morikawa, Tetsuro Lennikov, Anton Mukwaya, Anthony Schaupper, Mira Uehara, Osamu Takai, Rie Yoshida, Koki Sato, Jun Horie, Yukihiro Sakaguchi, Hiroyuki Wu, Ching-Zong Abiko, Yoshihiro Lagali, Neil Kitaichi, Nobuyoshi |
author_facet | Harada, Fumiya Morikawa, Tetsuro Lennikov, Anton Mukwaya, Anthony Schaupper, Mira Uehara, Osamu Takai, Rie Yoshida, Koki Sato, Jun Horie, Yukihiro Sakaguchi, Hiroyuki Wu, Ching-Zong Abiko, Yoshihiro Lagali, Neil Kitaichi, Nobuyoshi |
author_sort | Harada, Fumiya |
collection | PubMed |
description | PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. METHODS: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2)). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. RESULTS: Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p < 0.01), with significantly less NF-κB nucleus translocation (p < 0.001), and significantly fewer TUNEL cells (p < 0.01). Weaker DHE signals were detected in the nano-AST group (p < 0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. CONCLUSIONS: Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity. |
format | Online Article Text |
id | pubmed-5637851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56378512017-11-05 Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice Harada, Fumiya Morikawa, Tetsuro Lennikov, Anton Mukwaya, Anthony Schaupper, Mira Uehara, Osamu Takai, Rie Yoshida, Koki Sato, Jun Horie, Yukihiro Sakaguchi, Hiroyuki Wu, Ching-Zong Abiko, Yoshihiro Lagali, Neil Kitaichi, Nobuyoshi Oxid Med Cell Longev Research Article PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. METHODS: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2)). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. RESULTS: Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p < 0.01), with significantly less NF-κB nucleus translocation (p < 0.001), and significantly fewer TUNEL cells (p < 0.01). Weaker DHE signals were detected in the nano-AST group (p < 0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. CONCLUSIONS: Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity. Hindawi 2017 2017-09-28 /pmc/articles/PMC5637851/ /pubmed/29104724 http://dx.doi.org/10.1155/2017/1956104 Text en Copyright © 2017 Fumiya Harada et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Harada, Fumiya Morikawa, Tetsuro Lennikov, Anton Mukwaya, Anthony Schaupper, Mira Uehara, Osamu Takai, Rie Yoshida, Koki Sato, Jun Horie, Yukihiro Sakaguchi, Hiroyuki Wu, Ching-Zong Abiko, Yoshihiro Lagali, Neil Kitaichi, Nobuyoshi Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title | Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title_full | Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title_fullStr | Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title_full_unstemmed | Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title_short | Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice |
title_sort | protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637851/ https://www.ncbi.nlm.nih.gov/pubmed/29104724 http://dx.doi.org/10.1155/2017/1956104 |
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