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Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice

PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST ha...

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Autores principales: Harada, Fumiya, Morikawa, Tetsuro, Lennikov, Anton, Mukwaya, Anthony, Schaupper, Mira, Uehara, Osamu, Takai, Rie, Yoshida, Koki, Sato, Jun, Horie, Yukihiro, Sakaguchi, Hiroyuki, Wu, Ching-Zong, Abiko, Yoshihiro, Lagali, Neil, Kitaichi, Nobuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637851/
https://www.ncbi.nlm.nih.gov/pubmed/29104724
http://dx.doi.org/10.1155/2017/1956104
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author Harada, Fumiya
Morikawa, Tetsuro
Lennikov, Anton
Mukwaya, Anthony
Schaupper, Mira
Uehara, Osamu
Takai, Rie
Yoshida, Koki
Sato, Jun
Horie, Yukihiro
Sakaguchi, Hiroyuki
Wu, Ching-Zong
Abiko, Yoshihiro
Lagali, Neil
Kitaichi, Nobuyoshi
author_facet Harada, Fumiya
Morikawa, Tetsuro
Lennikov, Anton
Mukwaya, Anthony
Schaupper, Mira
Uehara, Osamu
Takai, Rie
Yoshida, Koki
Sato, Jun
Horie, Yukihiro
Sakaguchi, Hiroyuki
Wu, Ching-Zong
Abiko, Yoshihiro
Lagali, Neil
Kitaichi, Nobuyoshi
author_sort Harada, Fumiya
collection PubMed
description PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. METHODS: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2)). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. RESULTS: Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p < 0.01), with significantly less NF-κB nucleus translocation (p < 0.001), and significantly fewer TUNEL cells (p < 0.01). Weaker DHE signals were detected in the nano-AST group (p < 0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. CONCLUSIONS: Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity.
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spelling pubmed-56378512017-11-05 Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice Harada, Fumiya Morikawa, Tetsuro Lennikov, Anton Mukwaya, Anthony Schaupper, Mira Uehara, Osamu Takai, Rie Yoshida, Koki Sato, Jun Horie, Yukihiro Sakaguchi, Hiroyuki Wu, Ching-Zong Abiko, Yoshihiro Lagali, Neil Kitaichi, Nobuyoshi Oxid Med Cell Longev Research Article PURPOSE: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. METHODS: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2)). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. RESULTS: Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p < 0.01), with significantly less NF-κB nucleus translocation (p < 0.001), and significantly fewer TUNEL cells (p < 0.01). Weaker DHE signals were detected in the nano-AST group (p < 0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. CONCLUSIONS: Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity. Hindawi 2017 2017-09-28 /pmc/articles/PMC5637851/ /pubmed/29104724 http://dx.doi.org/10.1155/2017/1956104 Text en Copyright © 2017 Fumiya Harada et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Harada, Fumiya
Morikawa, Tetsuro
Lennikov, Anton
Mukwaya, Anthony
Schaupper, Mira
Uehara, Osamu
Takai, Rie
Yoshida, Koki
Sato, Jun
Horie, Yukihiro
Sakaguchi, Hiroyuki
Wu, Ching-Zong
Abiko, Yoshihiro
Lagali, Neil
Kitaichi, Nobuyoshi
Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title_full Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title_fullStr Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title_full_unstemmed Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title_short Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
title_sort protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637851/
https://www.ncbi.nlm.nih.gov/pubmed/29104724
http://dx.doi.org/10.1155/2017/1956104
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