Terminally Truncated Isopenicillin N Synthase Generates a Dithioester Product: Evidence for a Thioaldehyde Intermediate during Catalysis and a New Mode of Reaction for Non‐Heme Iron Oxidases

Isopenicillin N synthase (IPNS) catalyses the four‐electron oxidation of a tripeptide, l‐δ‐(α‐aminoadipoyl)‐l‐cysteinyl‐d‐valine (ACV), to give isopenicillin N (IPN), the first‐formed β‐lactam in penicillin and cephalosporin biosynthesis. IPNS catalysis is dependent upon an iron(II) cofactor and oxy...

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Detalles Bibliográficos
Autores principales: McNeill, Luke A., Brown, Toby J. N., Sami, Malkit, Clifton, Ian J., Burzlaff, Nicolai I., Claridge, Timothy D. W., Adlington, Robert M., Baldwin, Jack E., Rutledge, Peter J., Schofield, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637899/
https://www.ncbi.nlm.nih.gov/pubmed/28703303
http://dx.doi.org/10.1002/chem.201701592

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