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Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging
Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells. Although the small size and superb photophysical parameters of fluorescent-dyes offer unique advantages for high-resolution microscop...
Autores principales: | Schvartz, Tomer, Aloush, Noa, Goliand, Inna, Segal, Inbar, Nachmias, Dikla, Arbely, Eyal, Elia, Natalie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5638579/ https://www.ncbi.nlm.nih.gov/pubmed/28835375 http://dx.doi.org/10.1091/mbc.E17-03-0161 |
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