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Generation, Diversity Determination, and Application to Antibody Selection of a Human Naïve Fab Library

We constructed a large naïve human Fab library (3 × 10(10) colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and κ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically re...

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Detalles Bibliográficos
Autores principales:	Kim, Sangkyu, Park, Insoo, Park, Seung Gu, Cho, Seulki, Kim, Jin Hong, Ipper, Nagesh S., Choi, Sun Shim, Lee, Eung Suk, Hong, Hyo Jeong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Korean Society for Molecular and Cellular Biology 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5638773/ https://www.ncbi.nlm.nih.gov/pubmed/28927259 http://dx.doi.org/10.14348/molcells.2017.0106

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