Engineering species-like barriers to sexual reproduction

Controlling the exchange of genetic information between sexually reproducing populations has applications in agriculture, eradication of disease vectors, control of invasive species, and the safe study of emerging biotechnology applications. Here we introduce an approach to engineer a genetic barrie...

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Autores principales: Maselko, Maciej, Heinsch, Stephen C., Chacón, Jeremy M., Harcombe, William R., Smanski, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5638955/
https://www.ncbi.nlm.nih.gov/pubmed/29026112
http://dx.doi.org/10.1038/s41467-017-01007-3
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author Maselko, Maciej
Heinsch, Stephen C.
Chacón, Jeremy M.
Harcombe, William R.
Smanski, Michael J.
author_facet Maselko, Maciej
Heinsch, Stephen C.
Chacón, Jeremy M.
Harcombe, William R.
Smanski, Michael J.
author_sort Maselko, Maciej
collection PubMed
description Controlling the exchange of genetic information between sexually reproducing populations has applications in agriculture, eradication of disease vectors, control of invasive species, and the safe study of emerging biotechnology applications. Here we introduce an approach to engineer a genetic barrier to sexual reproduction between otherwise compatible populations. Programmable transcription factors drive lethal gene expression in hybrid offspring following undesired mating events. As a proof of concept, we target the ACT1 promoter of the model organism Saccharomyces cerevisiae using a dCas9-based transcriptional activator. Lethal overexpression of actin results from mating this engineered strain with a strain containing the wild-type ACT1 promoter.
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spelling pubmed-56389552017-10-17 Engineering species-like barriers to sexual reproduction Maselko, Maciej Heinsch, Stephen C. Chacón, Jeremy M. Harcombe, William R. Smanski, Michael J. Nat Commun Article Controlling the exchange of genetic information between sexually reproducing populations has applications in agriculture, eradication of disease vectors, control of invasive species, and the safe study of emerging biotechnology applications. Here we introduce an approach to engineer a genetic barrier to sexual reproduction between otherwise compatible populations. Programmable transcription factors drive lethal gene expression in hybrid offspring following undesired mating events. As a proof of concept, we target the ACT1 promoter of the model organism Saccharomyces cerevisiae using a dCas9-based transcriptional activator. Lethal overexpression of actin results from mating this engineered strain with a strain containing the wild-type ACT1 promoter. Nature Publishing Group UK 2017-10-12 /pmc/articles/PMC5638955/ /pubmed/29026112 http://dx.doi.org/10.1038/s41467-017-01007-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Maselko, Maciej
Heinsch, Stephen C.
Chacón, Jeremy M.
Harcombe, William R.
Smanski, Michael J.
Engineering species-like barriers to sexual reproduction
title Engineering species-like barriers to sexual reproduction
title_full Engineering species-like barriers to sexual reproduction
title_fullStr Engineering species-like barriers to sexual reproduction
title_full_unstemmed Engineering species-like barriers to sexual reproduction
title_short Engineering species-like barriers to sexual reproduction
title_sort engineering species-like barriers to sexual reproduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5638955/
https://www.ncbi.nlm.nih.gov/pubmed/29026112
http://dx.doi.org/10.1038/s41467-017-01007-3
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AT harcombewilliamr engineeringspecieslikebarrierstosexualreproduction
AT smanskimichaelj engineeringspecieslikebarrierstosexualreproduction

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