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Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method

Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig g...

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Autores principales: Cheong, Yeotaek, Oh, Changin, Lee, Kunkyu, Cho, Ki-hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639080/
https://www.ncbi.nlm.nih.gov/pubmed/27586468
http://dx.doi.org/10.4142/jvs.2017.18.3.283
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author Cheong, Yeotaek
Oh, Changin
Lee, Kunkyu
Cho, Ki-hyun
author_facet Cheong, Yeotaek
Oh, Changin
Lee, Kunkyu
Cho, Ki-hyun
author_sort Cheong, Yeotaek
collection PubMed
description Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 and MHP, whereas SS showed the opposite. Correlations between pathogens were assessed among 36 different pair combinations; only seven pairs showed statistically significant correlations. In conclusion, the oral fluid method could be a feasible way to detect various swine respiratory disease pathogens and, therefore, could complement current monitoring systems for respiratory diseases in the swine industry.
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spelling pubmed-56390802017-10-17 Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method Cheong, Yeotaek Oh, Changin Lee, Kunkyu Cho, Ki-hyun J Vet Sci Original Article Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 and MHP, whereas SS showed the opposite. Correlations between pathogens were assessed among 36 different pair combinations; only seven pairs showed statistically significant correlations. In conclusion, the oral fluid method could be a feasible way to detect various swine respiratory disease pathogens and, therefore, could complement current monitoring systems for respiratory diseases in the swine industry. The Korean Society of Veterinary Science 2017-09 2017-09-20 /pmc/articles/PMC5639080/ /pubmed/27586468 http://dx.doi.org/10.4142/jvs.2017.18.3.283 Text en © 2017 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cheong, Yeotaek
Oh, Changin
Lee, Kunkyu
Cho, Ki-hyun
Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title_full Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title_fullStr Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title_full_unstemmed Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title_short Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
title_sort survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in korea via oral fluid method
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639080/
https://www.ncbi.nlm.nih.gov/pubmed/27586468
http://dx.doi.org/10.4142/jvs.2017.18.3.283
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