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Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue
Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system re...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639087/ https://www.ncbi.nlm.nih.gov/pubmed/27586464 http://dx.doi.org/10.4142/jvs.2017.18.3.341 |
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author | Yasuda, Masahiro Kawabata, Jyunya Akieda-Asai, Sayaka Nasu, Tetsuo Date, Yukari |
author_facet | Yasuda, Masahiro Kawabata, Jyunya Akieda-Asai, Sayaka Nasu, Tetsuo Date, Yukari |
author_sort | Yasuda, Masahiro |
collection | PubMed |
description | Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages. Fat droplets were observed to accumulate in bovine mesenteric adipocytes cultured alone, whereas few fat droplets accumulated in adipocytes indirectly cocultured with macrophages. We also observed that GC-C was present in bovine mesenteric adipose tissue, and that fat droplet accumulation decreased after in vitro GNY administration. Expressions of mRNAs encoding lipogenic factors decreased significantly in adipocytes after either coculture or GNY administration. These results suggest that the GNY/GC-C system is part of the control system for lipid accumulation in bovine mesenteric adipose tissue. |
format | Online Article Text |
id | pubmed-5639087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56390872017-10-17 Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue Yasuda, Masahiro Kawabata, Jyunya Akieda-Asai, Sayaka Nasu, Tetsuo Date, Yukari J Vet Sci Original Article Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages. Fat droplets were observed to accumulate in bovine mesenteric adipocytes cultured alone, whereas few fat droplets accumulated in adipocytes indirectly cocultured with macrophages. We also observed that GC-C was present in bovine mesenteric adipose tissue, and that fat droplet accumulation decreased after in vitro GNY administration. Expressions of mRNAs encoding lipogenic factors decreased significantly in adipocytes after either coculture or GNY administration. These results suggest that the GNY/GC-C system is part of the control system for lipid accumulation in bovine mesenteric adipose tissue. The Korean Society of Veterinary Science 2017-09 2017-09-20 /pmc/articles/PMC5639087/ /pubmed/27586464 http://dx.doi.org/10.4142/jvs.2017.18.3.341 Text en © 2017 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yasuda, Masahiro Kawabata, Jyunya Akieda-Asai, Sayaka Nasu, Tetsuo Date, Yukari Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title | Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title_full | Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title_fullStr | Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title_full_unstemmed | Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title_short | Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
title_sort | guanylyl cyclase c and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639087/ https://www.ncbi.nlm.nih.gov/pubmed/27586464 http://dx.doi.org/10.4142/jvs.2017.18.3.341 |
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