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Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639275/ https://www.ncbi.nlm.nih.gov/pubmed/29042971 http://dx.doi.org/10.3892/etm.2017.4976 |
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author | Carvalho, Natalia S. Moron, Antonio F. Menon, Ramkumar Cavalheiro, Sergio Barbosa, Mauricio M. Milani, Herbene J. Ishigai, Marcia M. |
author_facet | Carvalho, Natalia S. Moron, Antonio F. Menon, Ramkumar Cavalheiro, Sergio Barbosa, Mauricio M. Milani, Herbene J. Ishigai, Marcia M. |
author_sort | Carvalho, Natalia S. |
collection | PubMed |
description | An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non-surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery. |
format | Online Article Text |
id | pubmed-5639275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56392752017-10-17 Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele Carvalho, Natalia S. Moron, Antonio F. Menon, Ramkumar Cavalheiro, Sergio Barbosa, Mauricio M. Milani, Herbene J. Ishigai, Marcia M. Exp Ther Med Articles An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non-surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery. D.A. Spandidos 2017-10 2017-08-21 /pmc/articles/PMC5639275/ /pubmed/29042971 http://dx.doi.org/10.3892/etm.2017.4976 Text en Copyright: © Carvalho et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Carvalho, Natalia S. Moron, Antonio F. Menon, Ramkumar Cavalheiro, Sergio Barbosa, Mauricio M. Milani, Herbene J. Ishigai, Marcia M. Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title | Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title_full | Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title_fullStr | Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title_full_unstemmed | Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title_short | Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
title_sort | histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639275/ https://www.ncbi.nlm.nih.gov/pubmed/29042971 http://dx.doi.org/10.3892/etm.2017.4976 |
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