Cargando…

Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery....

Descripción completa

Detalles Bibliográficos
Autores principales: Carvalho, Natalia S., Moron, Antonio F., Menon, Ramkumar, Cavalheiro, Sergio, Barbosa, Mauricio M., Milani, Herbene J., Ishigai, Marcia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639275/
https://www.ncbi.nlm.nih.gov/pubmed/29042971
http://dx.doi.org/10.3892/etm.2017.4976
_version_ 1783270854490062848
author Carvalho, Natalia S.
Moron, Antonio F.
Menon, Ramkumar
Cavalheiro, Sergio
Barbosa, Mauricio M.
Milani, Herbene J.
Ishigai, Marcia M.
author_facet Carvalho, Natalia S.
Moron, Antonio F.
Menon, Ramkumar
Cavalheiro, Sergio
Barbosa, Mauricio M.
Milani, Herbene J.
Ishigai, Marcia M.
author_sort Carvalho, Natalia S.
collection PubMed
description An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non-surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.
format Online
Article
Text
id pubmed-5639275
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-56392752017-10-17 Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele Carvalho, Natalia S. Moron, Antonio F. Menon, Ramkumar Cavalheiro, Sergio Barbosa, Mauricio M. Milani, Herbene J. Ishigai, Marcia M. Exp Ther Med Articles An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non-surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery. D.A. Spandidos 2017-10 2017-08-21 /pmc/articles/PMC5639275/ /pubmed/29042971 http://dx.doi.org/10.3892/etm.2017.4976 Text en Copyright: © Carvalho et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Carvalho, Natalia S.
Moron, Antonio F.
Menon, Ramkumar
Cavalheiro, Sergio
Barbosa, Mauricio M.
Milani, Herbene J.
Ishigai, Marcia M.
Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title_full Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title_fullStr Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title_full_unstemmed Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title_short Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
title_sort histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639275/
https://www.ncbi.nlm.nih.gov/pubmed/29042971
http://dx.doi.org/10.3892/etm.2017.4976
work_keys_str_mv AT carvalhonatalias histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT moronantoniof histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT menonramkumar histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT cavalheirosergio histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT barbosamauriciom histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT milaniherbenej histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele
AT ishigaimarciam histologicalevidenceofreparativeactivityinchorioamnioticmembranefollowingopenfetalsurgeryformyelomeningocele