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Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice
We have previously shown that human red blood cells (hRBCs) are subject to robust rejection by macrophages in immunodeficient mice. In this study, we found that mouse serum induces hRBC adherence to murine phagocytic cells, including professional phagocytic macrophages and neutrophils and non-profes...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639386/ https://www.ncbi.nlm.nih.gov/pubmed/28966117 http://dx.doi.org/10.1016/j.stemcr.2017.08.018 |
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author | Chen, Bing Fan, Wei Zou, Jun Zhang, Siwen He, Jin Shu, Chang Zhao, Guoqing Sun, Tianmeng Hu, Zheng Yang, Yong-Guang |
author_facet | Chen, Bing Fan, Wei Zou, Jun Zhang, Siwen He, Jin Shu, Chang Zhao, Guoqing Sun, Tianmeng Hu, Zheng Yang, Yong-Guang |
author_sort | Chen, Bing |
collection | PubMed |
description | We have previously shown that human red blood cells (hRBCs) are subject to robust rejection by macrophages in immunodeficient mice. In this study, we found that mouse serum induces hRBC adherence to murine phagocytic cells, including professional phagocytic macrophages and neutrophils and non-professional phagocytic endothelial cells. Complement was found to be responsible for mouse-serum-induced hRBC adherence to murine phagocytic cells. Although hRBC survival was not improved in NOD/SCID mice with complement depletion by cobra venom factor (CVF), CVF significantly prolonged hRBC survival in mice that were depleted of phagocytic macrophages by clodronate-liposomes. This combination treatment also synergistically improved hRBC reconstitution in human CD34(+) cell-grafted mice, offering a valuable model to examine human erythropoiesis and RBC function. These data indicate that complement, which might be dispensable for hRBC rejection by macrophages, is critical in hRBC rejection by other types of murine phagocytic cells, such as neutrophils and endothelial cells. |
format | Online Article Text |
id | pubmed-5639386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56393862017-10-20 Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice Chen, Bing Fan, Wei Zou, Jun Zhang, Siwen He, Jin Shu, Chang Zhao, Guoqing Sun, Tianmeng Hu, Zheng Yang, Yong-Guang Stem Cell Reports Report We have previously shown that human red blood cells (hRBCs) are subject to robust rejection by macrophages in immunodeficient mice. In this study, we found that mouse serum induces hRBC adherence to murine phagocytic cells, including professional phagocytic macrophages and neutrophils and non-professional phagocytic endothelial cells. Complement was found to be responsible for mouse-serum-induced hRBC adherence to murine phagocytic cells. Although hRBC survival was not improved in NOD/SCID mice with complement depletion by cobra venom factor (CVF), CVF significantly prolonged hRBC survival in mice that were depleted of phagocytic macrophages by clodronate-liposomes. This combination treatment also synergistically improved hRBC reconstitution in human CD34(+) cell-grafted mice, offering a valuable model to examine human erythropoiesis and RBC function. These data indicate that complement, which might be dispensable for hRBC rejection by macrophages, is critical in hRBC rejection by other types of murine phagocytic cells, such as neutrophils and endothelial cells. Elsevier 2017-09-28 /pmc/articles/PMC5639386/ /pubmed/28966117 http://dx.doi.org/10.1016/j.stemcr.2017.08.018 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Report Chen, Bing Fan, Wei Zou, Jun Zhang, Siwen He, Jin Shu, Chang Zhao, Guoqing Sun, Tianmeng Hu, Zheng Yang, Yong-Guang Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title | Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title_full | Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title_fullStr | Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title_full_unstemmed | Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title_short | Complement Depletion Improves Human Red Blood Cell Reconstitution in Immunodeficient Mice |
title_sort | complement depletion improves human red blood cell reconstitution in immunodeficient mice |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639386/ https://www.ncbi.nlm.nih.gov/pubmed/28966117 http://dx.doi.org/10.1016/j.stemcr.2017.08.018 |
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