Cargando…

An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells

Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated a library of over 200 endo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Harikumar, Arigela, Edupuganti, Raghu Ram, Sorek, Matan, Azad, Gajendra Kumar, Markoulaki, Styliani, Sehnalová, Petra, Legartová, Soňa, Bártová, Eva, Farkash-Amar, Shlomit, Jaenisch, Rudolf, Alon, Uri, Meshorer, Eran
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Resource
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639459/ https://www.ncbi.nlm.nih.gov/pubmed/28966122 http://dx.doi.org/10.1016/j.stemcr.2017.08.022

Ejemplares similares

Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation
por: Edupuganti, Raghu Ram, et al.
Publicado: (2017)

Can’t smell the virus: SARS-CoV-2, chromatin organization, and anosmia
por: Sorek, Matan, et al.
Publicado: (2022)

Impaired activation of transposable elements in SARS‐CoV‐2 infection
por: Sorek, Matan, et al.
Publicado: (2022)

Open chromatin structure in PolyQ disease-related genes: a potential mechanism for CAG repeat expansion in the normal human population
por: Sorek, Matan, et al.
Publicado: (2019)

Non-polyadenylated transcription in embryonic stem cells reveals novel non-coding RNA related to pluripotency and differentiation
por: Livyatan, Ilana, et al.
Publicado: (2013)

Embryonic Stem Cell Differentiation Is Regulated by SET through Interactions with p53 and β-Catenin
por: Harikumar, Arigela, et al.
Publicado: (2020)

Dynamic proteomics reveals bimodal protein dynamics of cancer cells in response to HSP90 inhibitor
por: Zimmer, Anat, et al.
Publicado: (2017)

A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress
por: Hanan, Mor, et al.
Publicado: (2020)

Molecular Criteria for Defining the Naive Human Pluripotent State
por: Theunissen, Thorold W., et al.
Publicado: (2016)

Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli
por: Krejčí, Jana, et al.
Publicado: (2017)

The Highest Density of Phosphorylated Histone H1 Appeared in Prophase and Prometaphase in Parallel with Reduced H3K9me3, and HDAC1 Depletion Increased H1.2/H1.3 and H1.4 Serine 38 Phosphorylation
por: Legartová, Soňa, et al.
Publicado: (2022)

Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells
por: Mattout, Anna, et al.
Publicado: (2015)

HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
por: Stixová, Lenka, et al.
Publicado: (2014)

Early sample tagging and pooling enables simultaneous SARS-CoV-2 detection and variant sequencing
por: Chappleboim, Alon, et al.
Publicado: (2021)

Systematic Determination of Replication Activity Type Highlights Interconnections between Replication, Chromatin Structure and Nuclear Localization
por: Farkash-Amar, Shlomit, et al.
Publicado: (2012)

Systematic identification of gene family regulators in mouse and human embryonic stem cells
por: Aaronson, Yair, et al.
Publicado: (2016)

Matched Developmental Timing of Donor Cells with the Host Is Crucial for Chimera Formation
por: Cohen, Malkiel A., et al.
Publicado: (2018)

A role of the 53BP1 protein in genome protection: structural and functional characteristics of 53BP1-dependent DNA repair
por: Bártová, Eva, et al.
Publicado: (2019)

H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions
por: Svobodová Kovaříková, Alena, et al.
Publicado: (2018)

Cell differentiation and aging accompanied by depletion of the ACE2 protein
por: Bártová, Eva, et al.
Publicado: (2020)

PRMT1 Arginine Methyltransferase Accumulates in Cytoplasmic Bodies that Respond to Selective Inhibition and DNA Damage
por: Suchánková, J., et al.
Publicado: (2014)

Dynamic Proteomics of Human Protein Level and Localization across the Cell Cycle
por: Farkash-Amar, Shlomit, et al.
Publicado: (2012)

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs
por: Roberts, Brock, et al.
Publicado: (2019)

Endogenously Tagged Rab Proteins: A Resource to Study Membrane Trafficking in Drosophila
por: Dunst, Sebastian, et al.
Publicado: (2015)

Noise Genetics: Inferring Protein Function by Correlating Phenotype with Protein Levels and Localization in Individual Human Cells
por: Farkash-Amar, Shlomit, et al.
Publicado: (2014)

Recruitment of Oct4 Protein to UV-Damaged Chromatin in Embryonic Stem Cells
por: Bártová, Eva, et al.
Publicado: (2011)

Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin
por: Stixová, Lenka, et al.
Publicado: (2011)

The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction
por: Legartová, Soňa, et al.
Publicado: (2021)

Mapping protein interactions of sodium channel Na(V)1.7 using epitope‐tagged gene‐targeted mice
por: Kanellopoulos, Alexandros H, et al.
Publicado: (2018)

Prediction of drug cocktail effects when the number of measurements is limited
por: Zimmer, Anat, et al.
Publicado: (2017)

Early recruitment of PARP-dependent m(8)A RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation
por: Legartová, Soňa, et al.
Publicado: (2022)

Higher chromatin mobility supports totipotency and precedes pluripotency in vivo
por: Bošković, Ana, et al.
Publicado: (2014)

N(6)-Adenosine Methylation in RNA and a Reduced m(3)G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions
por: Svobodová Kovaříková, Alena, et al.
Publicado: (2020)

Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos
por: Lye, Claire M., et al.
Publicado: (2014)

The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
por: Leader, Yodfat, et al.
Publicado: (2021)

DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88
por: Legartová, Soňa, et al.
Publicado: (2019)

Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta
por: Minner-Meinen, Rieke, et al.
Publicado: (2021)

mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells
por: Hatano, Tomoyuki, et al.
Publicado: (2022)

Internally tagged Vps10p-domain receptors reveal uptake of the neurotrophin BDNF
por: Klein, Marcel, et al.
Publicado: (2023)

Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster
por: Vigano, M. Alessandra, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_3pbir0bmsflhcvksa7egmo7kh4