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Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters

Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomi...

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Detalles Bibliográficos
Autores principales:	Chen, Yaoyao, Spitzer, Sonia, Agathou, Sylvia, Karadottir, Ragnhildur Thora, Smith, Austin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639479/ https://www.ncbi.nlm.nih.gov/pubmed/29020614 http://dx.doi.org/10.1016/j.stemcr.2017.09.005

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