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Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells

Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junc...

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Autores principales: Lee, Jung Bok, Balikov, Daniel A., Yang, Jae Won, Kim, Ki Seok, Park, Hun Kuk, Kim, Jeong Koo, Kwon, Il Keun, Bellan, Leon M., Sung, Hak-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639919/
https://www.ncbi.nlm.nih.gov/pubmed/29034114
http://dx.doi.org/10.3390/polym8010015
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author Lee, Jung Bok
Balikov, Daniel A.
Yang, Jae Won
Kim, Ki Seok
Park, Hun Kuk
Kim, Jeong Koo
Kwon, Il Keun
Bellan, Leon M.
Sung, Hak-Joon
author_facet Lee, Jung Bok
Balikov, Daniel A.
Yang, Jae Won
Kim, Ki Seok
Park, Hun Kuk
Kim, Jeong Koo
Kwon, Il Keun
Bellan, Leon M.
Sung, Hak-Joon
author_sort Lee, Jung Bok
collection PubMed
description Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junction formation, a hallmark of angiogenesis. Though continuous progress has been made, strategies to promote angiogenesis still rely on small molecule delivery or nuanced scaffold fabrication. As such, the recent paradigm shift from top-down to bottom-up approaches in tissue engineering necessitates development of polymer-based modular engineering tools to control angiogenesis. Here, we developed cationic nanocylinders (NCs) as inducers of cell–cell interaction and investigated their effect on angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Electrospun poly (l-lactic acid) (PLLA) fibers were aminolyzed to generate positively charged NCs. The aninolyzation time was changed to produce two different aspect ratios of NCs. When HUVECs were treated with NCs, the electrostatic interaction of cationic NCs with negatively charged plasma membranes promoted migration, permeability and tubulogenesis of HUVECs compared to no treatment. This effect was more profound when the higher aspect ratio NC was used. The results indicate these NCs can be used as a new tool for the bottom-up approach to promote angiogenesis.
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spelling pubmed-56399192017-10-13 Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells Lee, Jung Bok Balikov, Daniel A. Yang, Jae Won Kim, Ki Seok Park, Hun Kuk Kim, Jeong Koo Kwon, Il Keun Bellan, Leon M. Sung, Hak-Joon Polymers (Basel) Article Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junction formation, a hallmark of angiogenesis. Though continuous progress has been made, strategies to promote angiogenesis still rely on small molecule delivery or nuanced scaffold fabrication. As such, the recent paradigm shift from top-down to bottom-up approaches in tissue engineering necessitates development of polymer-based modular engineering tools to control angiogenesis. Here, we developed cationic nanocylinders (NCs) as inducers of cell–cell interaction and investigated their effect on angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Electrospun poly (l-lactic acid) (PLLA) fibers were aminolyzed to generate positively charged NCs. The aninolyzation time was changed to produce two different aspect ratios of NCs. When HUVECs were treated with NCs, the electrostatic interaction of cationic NCs with negatively charged plasma membranes promoted migration, permeability and tubulogenesis of HUVECs compared to no treatment. This effect was more profound when the higher aspect ratio NC was used. The results indicate these NCs can be used as a new tool for the bottom-up approach to promote angiogenesis. MDPI 2016-01-14 /pmc/articles/PMC5639919/ /pubmed/29034114 http://dx.doi.org/10.3390/polym8010015 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jung Bok
Balikov, Daniel A.
Yang, Jae Won
Kim, Ki Seok
Park, Hun Kuk
Kim, Jeong Koo
Kwon, Il Keun
Bellan, Leon M.
Sung, Hak-Joon
Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title_full Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title_fullStr Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title_full_unstemmed Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title_short Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
title_sort cationic nanocylinders promote angiogenic activities of endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639919/
https://www.ncbi.nlm.nih.gov/pubmed/29034114
http://dx.doi.org/10.3390/polym8010015
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