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Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study
Gastrointestinal (GI) symptoms are prevalent in autism spectrum disorder (ASD) but the pathophysiology is poorly understood. Imbalances in the enteric microbiome have been associated with ASD and can cause GI dysfunction potentially through disruption of mitochondrial function as microbiome metaboli...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640251/ https://www.ncbi.nlm.nih.gov/pubmed/29028817 http://dx.doi.org/10.1371/journal.pone.0186377 |
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author | Rose, Shannon Bennuri, Sirish C. Murray, Katherine F. Buie, Timothy Winter, Harland Frye, Richard Eugene |
author_facet | Rose, Shannon Bennuri, Sirish C. Murray, Katherine F. Buie, Timothy Winter, Harland Frye, Richard Eugene |
author_sort | Rose, Shannon |
collection | PubMed |
description | Gastrointestinal (GI) symptoms are prevalent in autism spectrum disorder (ASD) but the pathophysiology is poorly understood. Imbalances in the enteric microbiome have been associated with ASD and can cause GI dysfunction potentially through disruption of mitochondrial function as microbiome metabolites modulate mitochondrial function and mitochondrial dysfunction is highly associated with GI symptoms. In this study, we compared mitochondrial function in rectal and cecum biopsies under the assumption that certain microbiome metabolites, such as butyrate and propionic acid, are more abundant in the cecum as compared to the rectum. Rectal and cecum mucosal biopsies were collected during elective diagnostic colonoscopy. Using a single-blind case-control design, complex I and IV and citrate synthase activities and complex I-V protein quantity from 10 children with ASD, 10 children with Crohn’s disease and 10 neurotypical children with nonspecific GI complaints were measured. The protein for all complexes, except complex II, in the cecum as compared to the rectum was significantly higher in ASD samples as compared to other groups. For both rectal and cecum biopsies, ASD samples demonstrated higher complex I activity, but not complex IV or citrate synthase activity, compared to other groups. Mitochondrial function in the gut mucosa from children with ASD was found to be significantly different than other groups who manifested similar GI symptomatology suggesting a unique pathophysiology for GI symptoms in children with ASD. Abnormalities localized to the cecum suggest a role for imbalances in the microbiome, potentially in the production of butyrate, in children with ASD. |
format | Online Article Text |
id | pubmed-5640251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56402512017-10-30 Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study Rose, Shannon Bennuri, Sirish C. Murray, Katherine F. Buie, Timothy Winter, Harland Frye, Richard Eugene PLoS One Research Article Gastrointestinal (GI) symptoms are prevalent in autism spectrum disorder (ASD) but the pathophysiology is poorly understood. Imbalances in the enteric microbiome have been associated with ASD and can cause GI dysfunction potentially through disruption of mitochondrial function as microbiome metabolites modulate mitochondrial function and mitochondrial dysfunction is highly associated with GI symptoms. In this study, we compared mitochondrial function in rectal and cecum biopsies under the assumption that certain microbiome metabolites, such as butyrate and propionic acid, are more abundant in the cecum as compared to the rectum. Rectal and cecum mucosal biopsies were collected during elective diagnostic colonoscopy. Using a single-blind case-control design, complex I and IV and citrate synthase activities and complex I-V protein quantity from 10 children with ASD, 10 children with Crohn’s disease and 10 neurotypical children with nonspecific GI complaints were measured. The protein for all complexes, except complex II, in the cecum as compared to the rectum was significantly higher in ASD samples as compared to other groups. For both rectal and cecum biopsies, ASD samples demonstrated higher complex I activity, but not complex IV or citrate synthase activity, compared to other groups. Mitochondrial function in the gut mucosa from children with ASD was found to be significantly different than other groups who manifested similar GI symptomatology suggesting a unique pathophysiology for GI symptoms in children with ASD. Abnormalities localized to the cecum suggest a role for imbalances in the microbiome, potentially in the production of butyrate, in children with ASD. Public Library of Science 2017-10-13 /pmc/articles/PMC5640251/ /pubmed/29028817 http://dx.doi.org/10.1371/journal.pone.0186377 Text en © 2017 Rose et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rose, Shannon Bennuri, Sirish C. Murray, Katherine F. Buie, Timothy Winter, Harland Frye, Richard Eugene Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title | Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title_full | Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title_fullStr | Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title_full_unstemmed | Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title_short | Mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: A blinded case-control study |
title_sort | mitochondrial dysfunction in the gastrointestinal mucosa of children with autism: a blinded case-control study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640251/ https://www.ncbi.nlm.nih.gov/pubmed/29028817 http://dx.doi.org/10.1371/journal.pone.0186377 |
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