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Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels

Wave-like propagation of [Ca(2+)](i) increases is a remarkable intercellular communication characteristic in astrocyte networks, intercalating neural circuits and vasculature. Mechanically-induced [Ca(2+)](i) increases and their subsequent propagation to neighboring astrocytes in culture is a classi...

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Autores principales: Fujii, Yuki, Maekawa, Shohei, Morita, Mitsuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640625/
https://www.ncbi.nlm.nih.gov/pubmed/29030562
http://dx.doi.org/10.1038/s41598-017-13243-0
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author Fujii, Yuki
Maekawa, Shohei
Morita, Mitsuhiro
author_facet Fujii, Yuki
Maekawa, Shohei
Morita, Mitsuhiro
author_sort Fujii, Yuki
collection PubMed
description Wave-like propagation of [Ca(2+)](i) increases is a remarkable intercellular communication characteristic in astrocyte networks, intercalating neural circuits and vasculature. Mechanically-induced [Ca(2+)](i) increases and their subsequent propagation to neighboring astrocytes in culture is a classical model of astrocyte calcium wave and is known to be mediated by gap junction and extracellular ATP, but the role of each pathway remains unclear. Pharmacologic analysis of time-dependent distribution of [Ca(2+)](i) revealed three distinct [Ca(2+)](i) increases, the largest being in stimulated cells independent of extracellular Ca(2+) and inositol 1,4,5-trisphosphate-induced Ca(2+) release. In addition, persistent [Ca(2+)](i) increases were found to propagate rapidly via gap junctions in the proximal region, and transient [Ca(2+)](i) increases were found to propagate slowly via extracellular ATP in the distal region. Simultaneous imaging of astrocyte [Ca(2+)](i) and extracellular ATP, the latter of which was measured by an ATP sniffing cell, revealed that ATP was released within the proximal region by volume-regulated anion channel in a [Ca(2+)](i) independent manner. This detailed analysis of a classical model is the first to address the different contributions of two major pathways of calcium waves, gap junctions and extracellular ATP.
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spelling pubmed-56406252017-10-18 Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels Fujii, Yuki Maekawa, Shohei Morita, Mitsuhiro Sci Rep Article Wave-like propagation of [Ca(2+)](i) increases is a remarkable intercellular communication characteristic in astrocyte networks, intercalating neural circuits and vasculature. Mechanically-induced [Ca(2+)](i) increases and their subsequent propagation to neighboring astrocytes in culture is a classical model of astrocyte calcium wave and is known to be mediated by gap junction and extracellular ATP, but the role of each pathway remains unclear. Pharmacologic analysis of time-dependent distribution of [Ca(2+)](i) revealed three distinct [Ca(2+)](i) increases, the largest being in stimulated cells independent of extracellular Ca(2+) and inositol 1,4,5-trisphosphate-induced Ca(2+) release. In addition, persistent [Ca(2+)](i) increases were found to propagate rapidly via gap junctions in the proximal region, and transient [Ca(2+)](i) increases were found to propagate slowly via extracellular ATP in the distal region. Simultaneous imaging of astrocyte [Ca(2+)](i) and extracellular ATP, the latter of which was measured by an ATP sniffing cell, revealed that ATP was released within the proximal region by volume-regulated anion channel in a [Ca(2+)](i) independent manner. This detailed analysis of a classical model is the first to address the different contributions of two major pathways of calcium waves, gap junctions and extracellular ATP. Nature Publishing Group UK 2017-10-13 /pmc/articles/PMC5640625/ /pubmed/29030562 http://dx.doi.org/10.1038/s41598-017-13243-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fujii, Yuki
Maekawa, Shohei
Morita, Mitsuhiro
Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title_full Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title_fullStr Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title_full_unstemmed Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title_short Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels
title_sort astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of atp released from volume-regulated anion channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640625/
https://www.ncbi.nlm.nih.gov/pubmed/29030562
http://dx.doi.org/10.1038/s41598-017-13243-0
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AT moritamitsuhiro astrocytecalciumwavespropagateproximallybygapjunctionanddistallybyextracellulardiffusionofatpreleasedfromvolumeregulatedanionchannels