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Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several asp...

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Autores principales: Fernández-Castané, Alfred, Li, Hong, Thomas, Owen R. T., Overton, Tim W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640647/
https://www.ncbi.nlm.nih.gov/pubmed/29030621
http://dx.doi.org/10.1038/s41598-017-13414-z
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author Fernández-Castané, Alfred
Li, Hong
Thomas, Owen R. T.
Overton, Tim W.
author_facet Fernández-Castané, Alfred
Li, Hong
Thomas, Owen R. T.
Overton, Tim W.
author_sort Fernández-Castané, Alfred
collection PubMed
description Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several aspects of the physiology of the commonly-used MTB Magnetospirillum gryphiswaldense MSR-1. Flow cytometry is an optical technique that rapidly measures characteristics of individual bacteria within a culture, thereby allowing determination of population heterogeneity and also permitting direct analysis of bacteria. Scatter measurements were used to measure and compare bacterial size, shape and morphology. Membrane permeability and polarization were measured using the dyes propidium iodide and bis-(1,3-dibutylbarbituric acid) trimethine oxonol to determine the viability and ‘health’ of bacteria. Dyes were also used to determine changes in concentration of intracellular free iron and polyhydroxylakanoate (PHA), a bacterial energy storage polymer. These tools were then used to characterize the responses of MTB to different O(2) concentrations and iron-sufficient or iron-limited growth. Rapid analysis of MTB physiology will allow development of bioprocesses for the production of magnetosomes, and will increase understanding of this fascinating and useful group of bacteria.
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spelling pubmed-56406472017-10-18 Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1 Fernández-Castané, Alfred Li, Hong Thomas, Owen R. T. Overton, Tim W. Sci Rep Article Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several aspects of the physiology of the commonly-used MTB Magnetospirillum gryphiswaldense MSR-1. Flow cytometry is an optical technique that rapidly measures characteristics of individual bacteria within a culture, thereby allowing determination of population heterogeneity and also permitting direct analysis of bacteria. Scatter measurements were used to measure and compare bacterial size, shape and morphology. Membrane permeability and polarization were measured using the dyes propidium iodide and bis-(1,3-dibutylbarbituric acid) trimethine oxonol to determine the viability and ‘health’ of bacteria. Dyes were also used to determine changes in concentration of intracellular free iron and polyhydroxylakanoate (PHA), a bacterial energy storage polymer. These tools were then used to characterize the responses of MTB to different O(2) concentrations and iron-sufficient or iron-limited growth. Rapid analysis of MTB physiology will allow development of bioprocesses for the production of magnetosomes, and will increase understanding of this fascinating and useful group of bacteria. Nature Publishing Group UK 2017-10-13 /pmc/articles/PMC5640647/ /pubmed/29030621 http://dx.doi.org/10.1038/s41598-017-13414-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fernández-Castané, Alfred
Li, Hong
Thomas, Owen R. T.
Overton, Tim W.
Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title_full Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title_fullStr Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title_full_unstemmed Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title_short Flow cytometry as a rapid analytical tool to determine physiological responses to changing O(2) and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
title_sort flow cytometry as a rapid analytical tool to determine physiological responses to changing o(2) and iron concentration by magnetospirillum gryphiswaldense strain msr-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640647/
https://www.ncbi.nlm.nih.gov/pubmed/29030621
http://dx.doi.org/10.1038/s41598-017-13414-z
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