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Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position

Asymmetric positioning of the mitotic spindle is a fundamental process responsible for creating sibling cell size asymmetry; however, how the cortex causes the depolymerization of astral microtubules during asymmetric spindle positioning has remained elusive. Early ascidian embryos possess a large c...

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Autores principales: Costache, Vlad, Hebras, Celine, Pruliere, Gerard, Besnardeau, Lydia, Failla, Margaux, Copley, Richard R., Burgess, David, Chenevert, Janet, McDougall, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640700/
https://www.ncbi.nlm.nih.gov/pubmed/29030551
http://dx.doi.org/10.1038/s41467-017-01048-8
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author Costache, Vlad
Hebras, Celine
Pruliere, Gerard
Besnardeau, Lydia
Failla, Margaux
Copley, Richard R.
Burgess, David
Chenevert, Janet
McDougall, Alex
author_facet Costache, Vlad
Hebras, Celine
Pruliere, Gerard
Besnardeau, Lydia
Failla, Margaux
Copley, Richard R.
Burgess, David
Chenevert, Janet
McDougall, Alex
author_sort Costache, Vlad
collection PubMed
description Asymmetric positioning of the mitotic spindle is a fundamental process responsible for creating sibling cell size asymmetry; however, how the cortex causes the depolymerization of astral microtubules during asymmetric spindle positioning has remained elusive. Early ascidian embryos possess a large cortical subdomain of endoplasmic reticulum (ER) that causes asymmetric spindle positioning driving unequal cell division. Here we show that the microtubule depolymerase Kif2 localizes to this subdomain of cortical ER. Rapid live-cell imaging reveals that microtubules are less abundant in the subdomain of cortical ER. Inhibition of Kif2 function prevents the development of mitotic aster asymmetry and spindle pole movement towards the subdomain of cortical ER, whereas locally increasing microtubule depolymerization causes exaggerated asymmetric spindle positioning. This study shows that the microtubule depolymerase Kif2 is localized to a cortical subdomain of endoplasmic reticulum that is involved in asymmetric spindle positioning during unequal cell division.
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spelling pubmed-56407002017-10-18 Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position Costache, Vlad Hebras, Celine Pruliere, Gerard Besnardeau, Lydia Failla, Margaux Copley, Richard R. Burgess, David Chenevert, Janet McDougall, Alex Nat Commun Article Asymmetric positioning of the mitotic spindle is a fundamental process responsible for creating sibling cell size asymmetry; however, how the cortex causes the depolymerization of astral microtubules during asymmetric spindle positioning has remained elusive. Early ascidian embryos possess a large cortical subdomain of endoplasmic reticulum (ER) that causes asymmetric spindle positioning driving unequal cell division. Here we show that the microtubule depolymerase Kif2 localizes to this subdomain of cortical ER. Rapid live-cell imaging reveals that microtubules are less abundant in the subdomain of cortical ER. Inhibition of Kif2 function prevents the development of mitotic aster asymmetry and spindle pole movement towards the subdomain of cortical ER, whereas locally increasing microtubule depolymerization causes exaggerated asymmetric spindle positioning. This study shows that the microtubule depolymerase Kif2 is localized to a cortical subdomain of endoplasmic reticulum that is involved in asymmetric spindle positioning during unequal cell division. Nature Publishing Group UK 2017-10-13 /pmc/articles/PMC5640700/ /pubmed/29030551 http://dx.doi.org/10.1038/s41467-017-01048-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Costache, Vlad
Hebras, Celine
Pruliere, Gerard
Besnardeau, Lydia
Failla, Margaux
Copley, Richard R.
Burgess, David
Chenevert, Janet
McDougall, Alex
Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title_full Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title_fullStr Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title_full_unstemmed Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title_short Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
title_sort kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640700/
https://www.ncbi.nlm.nih.gov/pubmed/29030551
http://dx.doi.org/10.1038/s41467-017-01048-8
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