Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli

A new QepA4 variant was detected in an O86:H28 ST156-fimH38 Escherichia coli, showing a multidrug-resistance phenotype. PAβN inhibition of qepA4-harboring transconjugant resulted in increase of nalidixic acid accumulation. The qepA4 and catA1 genes were clustered in a 26.0-kp contig matching an IncF...

Descripción completa

Detalles Bibliográficos
Autores principales: Manageiro, Vera, Félix, David, Jones-Dias, Daniela, Sampaio, Daniel A., Vieira, Luís, Sancho, Luísa, Ferreira, Eugénia, Caniça, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640717/
https://www.ncbi.nlm.nih.gov/pubmed/29062302
http://dx.doi.org/10.3389/fmicb.2017.01899
_version_ 1783271085169442816
author Manageiro, Vera
Félix, David
Jones-Dias, Daniela
Sampaio, Daniel A.
Vieira, Luís
Sancho, Luísa
Ferreira, Eugénia
Caniça, Manuela
author_facet Manageiro, Vera
Félix, David
Jones-Dias, Daniela
Sampaio, Daniel A.
Vieira, Luís
Sancho, Luísa
Ferreira, Eugénia
Caniça, Manuela
author_sort Manageiro, Vera
collection PubMed
description A new QepA4 variant was detected in an O86:H28 ST156-fimH38 Escherichia coli, showing a multidrug-resistance phenotype. PAβN inhibition of qepA4-harboring transconjugant resulted in increase of nalidixic acid accumulation. The qepA4 and catA1 genes were clustered in a 26.0-kp contig matching an IncF-type plasmid, and containing a Tn21-type transposon with multiple mobile genetic elements. This QepA variant is worrisome because these determinants might facilitate the selection of higher-level resistance mutants, playing a role in the development of resistance, and/or confer higher-level resistance to fluoroquinolones in association with chromosomal mutations.
format Online
Article
Text
id pubmed-5640717
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-56407172017-10-23 Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli Manageiro, Vera Félix, David Jones-Dias, Daniela Sampaio, Daniel A. Vieira, Luís Sancho, Luísa Ferreira, Eugénia Caniça, Manuela Front Microbiol Microbiology A new QepA4 variant was detected in an O86:H28 ST156-fimH38 Escherichia coli, showing a multidrug-resistance phenotype. PAβN inhibition of qepA4-harboring transconjugant resulted in increase of nalidixic acid accumulation. The qepA4 and catA1 genes were clustered in a 26.0-kp contig matching an IncF-type plasmid, and containing a Tn21-type transposon with multiple mobile genetic elements. This QepA variant is worrisome because these determinants might facilitate the selection of higher-level resistance mutants, playing a role in the development of resistance, and/or confer higher-level resistance to fluoroquinolones in association with chromosomal mutations. Frontiers Media S.A. 2017-10-09 /pmc/articles/PMC5640717/ /pubmed/29062302 http://dx.doi.org/10.3389/fmicb.2017.01899 Text en Copyright © 2017 Manageiro, Félix, Jones-Dias, Sampaio, Vieira, Sancho, Ferreira and Caniça. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Manageiro, Vera
Félix, David
Jones-Dias, Daniela
Sampaio, Daniel A.
Vieira, Luís
Sancho, Luísa
Ferreira, Eugénia
Caniça, Manuela
Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title_full Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title_fullStr Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title_full_unstemmed Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title_short Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli
title_sort genetic background and expression of the new qepa4 gene variant recovered in clinical tem-1- and cmy-2-producing escherichia coli
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640717/
https://www.ncbi.nlm.nih.gov/pubmed/29062302
http://dx.doi.org/10.3389/fmicb.2017.01899
work_keys_str_mv AT manageirovera geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT felixdavid geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT jonesdiasdaniela geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT sampaiodaniela geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT vieiraluis geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT sancholuisa geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT ferreiraeugenia geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli
AT canicamanuela geneticbackgroundandexpressionofthenewqepa4genevariantrecoveredinclinicaltem1andcmy2producingescherichiacoli

Ejemplares similares