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Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate
BACKGROUND: The wound-healing process is very important for reducing complications after thulium laser resection of the prostate (TmLRP). The retinoic acid (RA) signaling pathway has been well studied in the wound-healing process of the skin and other organs. The goals of this study were to identify...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5641106/ https://www.ncbi.nlm.nih.gov/pubmed/29069763 http://dx.doi.org/10.18632/oncotarget.18238 |
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author | Yu, Dian-Jun Wang, Xing-Jie Shi, Yun-Feng Jiang, Chen-Yi Zhao, Rui-Zhe Zhu, Yi-Ping Chen, Li Yang, Yuan-Qing Sun, Xiao-Wen Xia, Shu-Jie |
author_facet | Yu, Dian-Jun Wang, Xing-Jie Shi, Yun-Feng Jiang, Chen-Yi Zhao, Rui-Zhe Zhu, Yi-Ping Chen, Li Yang, Yuan-Qing Sun, Xiao-Wen Xia, Shu-Jie |
author_sort | Yu, Dian-Jun |
collection | PubMed |
description | BACKGROUND: The wound-healing process is very important for reducing complications after thulium laser resection of the prostate (TmLRP). The retinoic acid (RA) signaling pathway has been well studied in the wound-healing process of the skin and other organs. The goals of this study were to identify the role of RA signaling in the repair of the prostate after TmLRP and to investigate the molecular mechanism of this process. RESULTS: Retinoic acid receptors (RARs) were present in the prostate, and their expression was increased after TmLRP. RARβ was expressed in the macrophages and may be related to the role of stromal cells in the wound-healing process. In vitro, RA enhanced the function of anti-inflammatory macrophages and promoted stromal cell activation and angiogenesis. Arg1 was also increased via RARβ after treatment with RA. MATERIALS AND METHODS: The expression of RARs was analyzed in vivo by immunohistochemistry (IHC), real time qPCR, and western blot analysis. THP-1 cells were co-treated with or without RA and stimulating factor and then assessed by ELISA and qPCR. The supernatants from these cells were cultured with stromal cells and vascular endothelial cells, and the effects on these cells were analyzed. CONCLUSIONS: We found that RA signaling was involved in the wound-healing process of the prostate after TmLRP. RA treated macrophages activated stromal cells and promoted angiogenesis. RARβ was the key isoform in this process. |
format | Online Article Text |
id | pubmed-5641106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56411062017-10-24 Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate Yu, Dian-Jun Wang, Xing-Jie Shi, Yun-Feng Jiang, Chen-Yi Zhao, Rui-Zhe Zhu, Yi-Ping Chen, Li Yang, Yuan-Qing Sun, Xiao-Wen Xia, Shu-Jie Oncotarget Research Paper BACKGROUND: The wound-healing process is very important for reducing complications after thulium laser resection of the prostate (TmLRP). The retinoic acid (RA) signaling pathway has been well studied in the wound-healing process of the skin and other organs. The goals of this study were to identify the role of RA signaling in the repair of the prostate after TmLRP and to investigate the molecular mechanism of this process. RESULTS: Retinoic acid receptors (RARs) were present in the prostate, and their expression was increased after TmLRP. RARβ was expressed in the macrophages and may be related to the role of stromal cells in the wound-healing process. In vitro, RA enhanced the function of anti-inflammatory macrophages and promoted stromal cell activation and angiogenesis. Arg1 was also increased via RARβ after treatment with RA. MATERIALS AND METHODS: The expression of RARs was analyzed in vivo by immunohistochemistry (IHC), real time qPCR, and western blot analysis. THP-1 cells were co-treated with or without RA and stimulating factor and then assessed by ELISA and qPCR. The supernatants from these cells were cultured with stromal cells and vascular endothelial cells, and the effects on these cells were analyzed. CONCLUSIONS: We found that RA signaling was involved in the wound-healing process of the prostate after TmLRP. RA treated macrophages activated stromal cells and promoted angiogenesis. RARβ was the key isoform in this process. Impact Journals LLC 2017-05-26 /pmc/articles/PMC5641106/ /pubmed/29069763 http://dx.doi.org/10.18632/oncotarget.18238 Text en Copyright: © 2017 Yu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Yu, Dian-Jun Wang, Xing-Jie Shi, Yun-Feng Jiang, Chen-Yi Zhao, Rui-Zhe Zhu, Yi-Ping Chen, Li Yang, Yuan-Qing Sun, Xiao-Wen Xia, Shu-Jie Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title | Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title_full | Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title_fullStr | Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title_full_unstemmed | Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title_short | Macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
title_sort | macrophages are targets of retinoic acid signaling during the wound-healing process after thulium laser resection of the prostate |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5641106/ https://www.ncbi.nlm.nih.gov/pubmed/29069763 http://dx.doi.org/10.18632/oncotarget.18238 |
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