Muscle ion transporters and antioxidative proteins have different adaptive potential in arm than in leg skeletal muscle with exercise training

It was evaluated whether upper‐body compared to lower‐body musculature exhibits a different phenotype in relation to capacity for handling reactive oxygen species (ROS), H(+), La(−), Na(+), K(+) and also whether it differs in adaptive potential to exercise training. Eighty‐three sedentary premenopausal women aged 45 ± 6 years (mean ± SD) were randomized into a high‐intensity intermittent swimming group (HIS, n = 21), a moderate‐intensity swimming group (MOS, n = 21), a soccer group (SOC, n = 21), or a control group (CON, n = 20). Intervention groups completed three weekly training sessions for 15 weeks, and pre‐ and postintervention biopsies were obtained from deltoideus and vastus lateralis muscle. Before training, monocarboxylate transporter 4 (MCT4), Na(+)/K(+) pump α (2), and superoxide dismutase 2 (SOD2) expressions were lower (P < 0.05) in m. deltoideus than in m. vastus lateralis, whereas deltoid had higher (P < 0.05) Na(+)/H(+) exchanger 1 (NHE1) expression. As a result of training, Na(+)/K(+) pump α (2) isoform expression was elevated only in deltoideus muscle, while upregulation (P < 0.05) of the α (1) and β (1) subunits, phospholemman (FXYD1), NHE1, and superoxide dismutase 1 expression occurred exclusively in vastus lateralis muscle. The increased (P < 0.05) expression of MCT4 and SOD2 in deltoid muscle after HIS and vastus lateralis muscle after SOC were similar. In conclusion, arm musculature displays lower basal ROS, La(−), K(+) handling capability but higher Na(+)‐dependent H(+) extrusion capacity than leg musculature. Training‐induced changes in the ion‐transporting and antioxidant proteins clearly differed between muscle groups.