The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer

Several long non-coding RNAs (lncRNAs) play important roles in the regulation of liver metastasis in colorectal cancer (CRC) patients. We previously described the potential involvement of HOMEOBOX A11 (HOXA11) antisense RNA (HOXA11-AS), miR-125a-5p, and peptidyl arginine deiminase 2 (PADI2) in promo...

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Autores principales: Chen, Dong, Sun, Qiang, Zhang, Lufei, Zhou, Xiaohu, Cheng, Xiaofei, Zhou, Dongkai, Ye, Feng, Lin, Jianjiang, Wang, Weilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5642583/
https://www.ncbi.nlm.nih.gov/pubmed/29050308
http://dx.doi.org/10.18632/oncotarget.19956
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author Chen, Dong
Sun, Qiang
Zhang, Lufei
Zhou, Xiaohu
Cheng, Xiaofei
Zhou, Dongkai
Ye, Feng
Lin, Jianjiang
Wang, Weilin
author_facet Chen, Dong
Sun, Qiang
Zhang, Lufei
Zhou, Xiaohu
Cheng, Xiaofei
Zhou, Dongkai
Ye, Feng
Lin, Jianjiang
Wang, Weilin
author_sort Chen, Dong
collection PubMed
description Several long non-coding RNAs (lncRNAs) play important roles in the regulation of liver metastasis in colorectal cancer (CRC) patients. We previously described the potential involvement of HOMEOBOX A11 (HOXA11) antisense RNA (HOXA11-AS), miR-125a-5p, and peptidyl arginine deiminase 2 (PADI2) in promoting liver metastasis in CRC patients. In the present study, we verified the significant upregulation of HOXA11-AS and PADI2, as well as the downregulation of miR-125a-5p, in CRC patients with liver metastasis. Overexpression and knockdown studies of HOXA11-AS or PADI2, as well as gain-/loss-of-function studies of miR-125a-5p, revealed a positive correlation between HOXA11-AS and PADI2 and a negative correlation with miR-125a-5p in the regulation of liver metastasis in CRC cell lines. Overall, we conclude that HOXA11-AS promotes liver metastasis in CRC by functioning as a miR-125a-5p sponge and describe a novel HOXA11-AS–miR-125a-5p–PADI2 regulatory network involved in CRC liver metastasis.
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spelling pubmed-56425832017-10-18 The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer Chen, Dong Sun, Qiang Zhang, Lufei Zhou, Xiaohu Cheng, Xiaofei Zhou, Dongkai Ye, Feng Lin, Jianjiang Wang, Weilin Oncotarget Research Paper Several long non-coding RNAs (lncRNAs) play important roles in the regulation of liver metastasis in colorectal cancer (CRC) patients. We previously described the potential involvement of HOMEOBOX A11 (HOXA11) antisense RNA (HOXA11-AS), miR-125a-5p, and peptidyl arginine deiminase 2 (PADI2) in promoting liver metastasis in CRC patients. In the present study, we verified the significant upregulation of HOXA11-AS and PADI2, as well as the downregulation of miR-125a-5p, in CRC patients with liver metastasis. Overexpression and knockdown studies of HOXA11-AS or PADI2, as well as gain-/loss-of-function studies of miR-125a-5p, revealed a positive correlation between HOXA11-AS and PADI2 and a negative correlation with miR-125a-5p in the regulation of liver metastasis in CRC cell lines. Overall, we conclude that HOXA11-AS promotes liver metastasis in CRC by functioning as a miR-125a-5p sponge and describe a novel HOXA11-AS–miR-125a-5p–PADI2 regulatory network involved in CRC liver metastasis. Impact Journals LLC 2017-08-03 /pmc/articles/PMC5642583/ /pubmed/29050308 http://dx.doi.org/10.18632/oncotarget.19956 Text en Copyright: © 2017 Chen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, Dong
Sun, Qiang
Zhang, Lufei
Zhou, Xiaohu
Cheng, Xiaofei
Zhou, Dongkai
Ye, Feng
Lin, Jianjiang
Wang, Weilin
The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title_full The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title_fullStr The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title_full_unstemmed The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title_short The lncRNA HOXA11-AS functions as a competing endogenous RNA to regulate PADI2 expression by sponging miR-125a-5p in liver metastasis of colorectal cancer
title_sort lncrna hoxa11-as functions as a competing endogenous rna to regulate padi2 expression by sponging mir-125a-5p in liver metastasis of colorectal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5642583/
https://www.ncbi.nlm.nih.gov/pubmed/29050308
http://dx.doi.org/10.18632/oncotarget.19956
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