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An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease

Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children below 5 years of age in endemic areas, and is a primary cause of diarrhoea in travellers visiting developing countries. Epidemiological analysis of E. coli pathovars is traditionally carried out based on the results of...

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Autores principales: Iguchi, Atsushi, von Mentzer, Astrid, Kikuchi, Taisei, Thomson, Nicholas R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643014/
https://www.ncbi.nlm.nih.gov/pubmed/29114400
http://dx.doi.org/10.1099/mgen.0.000121
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author Iguchi, Atsushi
von Mentzer, Astrid
Kikuchi, Taisei
Thomson, Nicholas R.
author_facet Iguchi, Atsushi
von Mentzer, Astrid
Kikuchi, Taisei
Thomson, Nicholas R.
author_sort Iguchi, Atsushi
collection PubMed
description Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children below 5 years of age in endemic areas, and is a primary cause of diarrhoea in travellers visiting developing countries. Epidemiological analysis of E. coli pathovars is traditionally carried out based on the results of serotyping. However, genomic analysis of a global ETEC collection of 362 isolates taken from patients revealed nine novel O-antigen biosynthesis gene clusters that were previously unrecognized, and have collectively been called unclassified. When put in the context of all isolates sequenced, one of the novel O-genotypes, OgN5, was found to be the second most common ETEC O-genotype causing disease, after O6, in a globally representative ETEC collection. It’s also clear that ETEC OgN5 isolates have spread globally. These novel O-genotypes have now been included in our comprehensive O-genotyping scheme, and can be detected using a PCR-based and an in silico typing method. This will assist in epidemiological studies, as well as in ETEC vaccine development.
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spelling pubmed-56430142017-11-07 An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease Iguchi, Atsushi von Mentzer, Astrid Kikuchi, Taisei Thomson, Nicholas R. Microb Genom Research Article Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children below 5 years of age in endemic areas, and is a primary cause of diarrhoea in travellers visiting developing countries. Epidemiological analysis of E. coli pathovars is traditionally carried out based on the results of serotyping. However, genomic analysis of a global ETEC collection of 362 isolates taken from patients revealed nine novel O-antigen biosynthesis gene clusters that were previously unrecognized, and have collectively been called unclassified. When put in the context of all isolates sequenced, one of the novel O-genotypes, OgN5, was found to be the second most common ETEC O-genotype causing disease, after O6, in a globally representative ETEC collection. It’s also clear that ETEC OgN5 isolates have spread globally. These novel O-genotypes have now been included in our comprehensive O-genotyping scheme, and can be detected using a PCR-based and an in silico typing method. This will assist in epidemiological studies, as well as in ETEC vaccine development. Microbiology Society 2017-07-03 /pmc/articles/PMC5643014/ /pubmed/29114400 http://dx.doi.org/10.1099/mgen.0.000121 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Iguchi, Atsushi
von Mentzer, Astrid
Kikuchi, Taisei
Thomson, Nicholas R.
An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title_full An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title_fullStr An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title_full_unstemmed An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title_short An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease
title_sort untypeable enterotoxigenic escherichia coli represents one of the dominant types causing human disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643014/
https://www.ncbi.nlm.nih.gov/pubmed/29114400
http://dx.doi.org/10.1099/mgen.0.000121
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