Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using...

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Detalles Bibliográficos
Autores principales: Williams, Maggie R., Stedtfeld, Robert D., Engle, Cathrine, Salach, Paul, Fakher, Umama, Stedtfeld, Tiffany, Dreelin, Erin, Stevenson, R. Jan, Latimore, Jo, Hashsham, Syed A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643059/
https://www.ncbi.nlm.nih.gov/pubmed/29036210
http://dx.doi.org/10.1371/journal.pone.0186462

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