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Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice

C1 esterase inhibitor (C1-INH) can inhibit multiple pathways (complement, contact-kinin, coagulation, and fibrinolysis) that are all implicated in the pathophysiology of asthma. We explored the effect of human plasma-derived C1-INH on allergic lung inflammation in a house dust mite (HDM) induced ast...

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Autores principales: Stroo, Ingrid, Yang, Jack, Anas, Adam A., de Boer, J. Daan, van Mierlo, Gerard, Roem, Dorina, Wouters, Diana, Engel, Ruchira, Roelofs, Joris J. T. H., van ‘t Veer, Cornelis, van der Poll, Tom, Zeerleder, Sacha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643136/
https://www.ncbi.nlm.nih.gov/pubmed/29036225
http://dx.doi.org/10.1371/journal.pone.0186652
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author Stroo, Ingrid
Yang, Jack
Anas, Adam A.
de Boer, J. Daan
van Mierlo, Gerard
Roem, Dorina
Wouters, Diana
Engel, Ruchira
Roelofs, Joris J. T. H.
van ‘t Veer, Cornelis
van der Poll, Tom
Zeerleder, Sacha
author_facet Stroo, Ingrid
Yang, Jack
Anas, Adam A.
de Boer, J. Daan
van Mierlo, Gerard
Roem, Dorina
Wouters, Diana
Engel, Ruchira
Roelofs, Joris J. T. H.
van ‘t Veer, Cornelis
van der Poll, Tom
Zeerleder, Sacha
author_sort Stroo, Ingrid
collection PubMed
description C1 esterase inhibitor (C1-INH) can inhibit multiple pathways (complement, contact-kinin, coagulation, and fibrinolysis) that are all implicated in the pathophysiology of asthma. We explored the effect of human plasma-derived C1-INH on allergic lung inflammation in a house dust mite (HDM) induced asthma mouse model by daily administration of C1-INH (15 U) during the challenge phase. NaCl and HDM exposed mice had comparable plasma C1-INH levels, while bronchoalveolar lavage fluid (BALF) levels were increased in HDM exposed mice coinciding with slightly reduced activation of complement (C5a). C1-INH treatment reduced Th(2) response and enhanced HDM-specific IgG(1). Influx of eosinophils in BALF or lung, pulmonary damage, mucus production, procoagulant response or plasma leakage in BALF was similar in both groups. In conclusion, C1-INH dampens Th2 responses during HDM induced allergic lung inflammation.
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spelling pubmed-56431362017-10-30 Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice Stroo, Ingrid Yang, Jack Anas, Adam A. de Boer, J. Daan van Mierlo, Gerard Roem, Dorina Wouters, Diana Engel, Ruchira Roelofs, Joris J. T. H. van ‘t Veer, Cornelis van der Poll, Tom Zeerleder, Sacha PLoS One Research Article C1 esterase inhibitor (C1-INH) can inhibit multiple pathways (complement, contact-kinin, coagulation, and fibrinolysis) that are all implicated in the pathophysiology of asthma. We explored the effect of human plasma-derived C1-INH on allergic lung inflammation in a house dust mite (HDM) induced asthma mouse model by daily administration of C1-INH (15 U) during the challenge phase. NaCl and HDM exposed mice had comparable plasma C1-INH levels, while bronchoalveolar lavage fluid (BALF) levels were increased in HDM exposed mice coinciding with slightly reduced activation of complement (C5a). C1-INH treatment reduced Th(2) response and enhanced HDM-specific IgG(1). Influx of eosinophils in BALF or lung, pulmonary damage, mucus production, procoagulant response or plasma leakage in BALF was similar in both groups. In conclusion, C1-INH dampens Th2 responses during HDM induced allergic lung inflammation. Public Library of Science 2017-10-16 /pmc/articles/PMC5643136/ /pubmed/29036225 http://dx.doi.org/10.1371/journal.pone.0186652 Text en © 2017 Stroo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Stroo, Ingrid
Yang, Jack
Anas, Adam A.
de Boer, J. Daan
van Mierlo, Gerard
Roem, Dorina
Wouters, Diana
Engel, Ruchira
Roelofs, Joris J. T. H.
van ‘t Veer, Cornelis
van der Poll, Tom
Zeerleder, Sacha
Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title_full Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title_fullStr Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title_full_unstemmed Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title_short Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
title_sort human plasma-derived c1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643136/
https://www.ncbi.nlm.nih.gov/pubmed/29036225
http://dx.doi.org/10.1371/journal.pone.0186652
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