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Quantitative Characterization of the T Cell Receptor Repertoire of Naïve and Memory Subsets Using an Integrated Experimental and Computational Pipeline Which Is Robust, Economical, and Versatile

The T cell receptor (TCR) repertoire can provide a personalized biomarker for infectious and non-infectious diseases. We describe a protocol for amplifying, sequencing, and analyzing TCRs which is robust, sensitive, and versatile. The key experimental step is ligation of a single-stranded oligonucle...

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Detalles Bibliográficos
Autores principales:	Oakes, Theres, Heather, James M., Best, Katharine, Byng-Maddick, Rachel, Husovsky, Connor, Ismail, Mazlina, Joshi, Kroopa, Maxwell, Gavin, Noursadeghi, Mahdad, Riddell, Natalie, Ruehl, Tabea, Turner, Carolin T., Uddin, Imran, Chain, Benny
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643411/ https://www.ncbi.nlm.nih.gov/pubmed/29075258 http://dx.doi.org/10.3389/fimmu.2017.01267

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