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Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization

There are large variations in the reported efficiency of gold nanoparticle (GNP) radiosensitization. We have previously reported on a predictive model, which accounts for the detailed Auger and photoelectron tracks to calculate the cell survival probability. After validating our model using PC-3 cel...

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Autores principales: Lechtman, Eli, Pignol, Jean-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643548/
https://www.ncbi.nlm.nih.gov/pubmed/29038517
http://dx.doi.org/10.1038/s41598-017-13736-y
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author Lechtman, Eli
Pignol, Jean-Philippe
author_facet Lechtman, Eli
Pignol, Jean-Philippe
author_sort Lechtman, Eli
collection PubMed
description There are large variations in the reported efficiency of gold nanoparticle (GNP) radiosensitization. We have previously reported on a predictive model, which accounts for the detailed Auger and photoelectron tracks to calculate the cell survival probability. After validating our model using PC-3 cells incubated with 2 mg/ml of 30 nm GNPs and irradiated with 100 kVp or 300 kVp beams, we evaluated the interplay between photon energy, GNP size (1.9 and 100 nm) and sub-cellular localization. Experiments were in excellent agreement with the model. In predictive modeling, using a 100 kVp source and 1.9 nm nanoparticles, GNP localization had a significant impact on cell survival. A sensitizer enhancement ratio of 1.34 was achieved when GNPs were localized outside the cells, increasing to 2.56 when GNPs were also distributed in the cytoplasm and nucleus. Using a 300 kVp source, which emits photons mainly above the gold K-edge, the dependence on GNP localization and size was barely detectable, since long ranged electrons dominate the energy deposition. In summary, achieving intracellular uptake with targeted-GNPs can significantly enhance radiosensitization for photon energies below the gold K-edge, where Auger electrons contribute significantly to the local energy deposition. For higher energies, this is much less important.
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spelling pubmed-56435482017-10-19 Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization Lechtman, Eli Pignol, Jean-Philippe Sci Rep Article There are large variations in the reported efficiency of gold nanoparticle (GNP) radiosensitization. We have previously reported on a predictive model, which accounts for the detailed Auger and photoelectron tracks to calculate the cell survival probability. After validating our model using PC-3 cells incubated with 2 mg/ml of 30 nm GNPs and irradiated with 100 kVp or 300 kVp beams, we evaluated the interplay between photon energy, GNP size (1.9 and 100 nm) and sub-cellular localization. Experiments were in excellent agreement with the model. In predictive modeling, using a 100 kVp source and 1.9 nm nanoparticles, GNP localization had a significant impact on cell survival. A sensitizer enhancement ratio of 1.34 was achieved when GNPs were localized outside the cells, increasing to 2.56 when GNPs were also distributed in the cytoplasm and nucleus. Using a 300 kVp source, which emits photons mainly above the gold K-edge, the dependence on GNP localization and size was barely detectable, since long ranged electrons dominate the energy deposition. In summary, achieving intracellular uptake with targeted-GNPs can significantly enhance radiosensitization for photon energies below the gold K-edge, where Auger electrons contribute significantly to the local energy deposition. For higher energies, this is much less important. Nature Publishing Group UK 2017-10-16 /pmc/articles/PMC5643548/ /pubmed/29038517 http://dx.doi.org/10.1038/s41598-017-13736-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lechtman, Eli
Pignol, Jean-Philippe
Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title_full Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title_fullStr Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title_full_unstemmed Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title_short Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
title_sort interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643548/
https://www.ncbi.nlm.nih.gov/pubmed/29038517
http://dx.doi.org/10.1038/s41598-017-13736-y
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