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Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice
The phenotypic characterization of self-reactive B cells producing autoantibodies is one of the challenges to get further insight in the physiopathology of autoimmune diseases. We took advantage of our previously developed flow cytometry method, using labeled nucleosomes, prominent autoantigens in s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643551/ https://www.ncbi.nlm.nih.gov/pubmed/29038433 http://dx.doi.org/10.1038/s41598-017-13422-z |
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author | Gies, Vincent Bouis, Delphine Martin, Mickaël Pasquali, Jean-L. Martin, Thierry Korganow, Anne-S. Soulas-Sprauel, Pauline |
author_facet | Gies, Vincent Bouis, Delphine Martin, Mickaël Pasquali, Jean-L. Martin, Thierry Korganow, Anne-S. Soulas-Sprauel, Pauline |
author_sort | Gies, Vincent |
collection | PubMed |
description | The phenotypic characterization of self-reactive B cells producing autoantibodies is one of the challenges to get further insight in the physiopathology of autoimmune diseases. We took advantage of our previously developed flow cytometry method, using labeled nucleosomes, prominent autoantigens in systemic lupus erythematosus, to analyze the phenotype of self-reactive B cells in the anti-DNA B6.56R mouse model. We showed that splenic anti-nucleosome B cells express mostly kappa light chains and harbor a marginal zone phenotype. Moreover, these autoreactive B cells fail to acquire a germinal center phenotype and are less abundant in the transitional T3 compartment. In conclusion, the direct detection of autoreactive B cells helped determine their phenotypic characteristics and provided a more direct insight into the B cell tolerance process in B6.56R mice. This method constitutes an interesting new tool to study the mechanisms of B cell tolerance breakdown in B6.56R mice crossed with autoimmune prone models. |
format | Online Article Text |
id | pubmed-5643551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56435512017-10-19 Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice Gies, Vincent Bouis, Delphine Martin, Mickaël Pasquali, Jean-L. Martin, Thierry Korganow, Anne-S. Soulas-Sprauel, Pauline Sci Rep Article The phenotypic characterization of self-reactive B cells producing autoantibodies is one of the challenges to get further insight in the physiopathology of autoimmune diseases. We took advantage of our previously developed flow cytometry method, using labeled nucleosomes, prominent autoantigens in systemic lupus erythematosus, to analyze the phenotype of self-reactive B cells in the anti-DNA B6.56R mouse model. We showed that splenic anti-nucleosome B cells express mostly kappa light chains and harbor a marginal zone phenotype. Moreover, these autoreactive B cells fail to acquire a germinal center phenotype and are less abundant in the transitional T3 compartment. In conclusion, the direct detection of autoreactive B cells helped determine their phenotypic characteristics and provided a more direct insight into the B cell tolerance process in B6.56R mice. This method constitutes an interesting new tool to study the mechanisms of B cell tolerance breakdown in B6.56R mice crossed with autoimmune prone models. Nature Publishing Group UK 2017-10-16 /pmc/articles/PMC5643551/ /pubmed/29038433 http://dx.doi.org/10.1038/s41598-017-13422-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gies, Vincent Bouis, Delphine Martin, Mickaël Pasquali, Jean-L. Martin, Thierry Korganow, Anne-S. Soulas-Sprauel, Pauline Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title | Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title_full | Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title_fullStr | Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title_full_unstemmed | Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title_short | Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice |
title_sort | phenotyping of autoreactive b cells with labeled nucleosomes in 56r transgenic mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643551/ https://www.ncbi.nlm.nih.gov/pubmed/29038433 http://dx.doi.org/10.1038/s41598-017-13422-z |
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