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Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, sub...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644692/ https://www.ncbi.nlm.nih.gov/pubmed/28409238 http://dx.doi.org/10.1007/s10482-017-0872-0 |
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author | Zamlynska, Katarzyna Komaniecka, Iwona Zebracki, Kamil Mazur, Andrzej Sroka-Bartnicka, Anna Choma, Adam |
author_facet | Zamlynska, Katarzyna Komaniecka, Iwona Zebracki, Kamil Mazur, Andrzej Sroka-Bartnicka, Anna Choma, Adam |
author_sort | Zamlynska, Katarzyna |
collection | PubMed |
description | The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes. |
format | Online Article Text |
id | pubmed-5644692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-56446922017-10-27 Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide Zamlynska, Katarzyna Komaniecka, Iwona Zebracki, Kamil Mazur, Andrzej Sroka-Bartnicka, Anna Choma, Adam Antonie Van Leeuwenhoek Original Paper The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes. Springer International Publishing 2017-04-13 2017 /pmc/articles/PMC5644692/ /pubmed/28409238 http://dx.doi.org/10.1007/s10482-017-0872-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Zamlynska, Katarzyna Komaniecka, Iwona Zebracki, Kamil Mazur, Andrzej Sroka-Bartnicka, Anna Choma, Adam Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title | Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title_full | Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title_fullStr | Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title_full_unstemmed | Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title_short | Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide |
title_sort | studies on lipid a isolated from phyllobacterium trifolii petp02(t) lipopolysaccharide |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644692/ https://www.ncbi.nlm.nih.gov/pubmed/28409238 http://dx.doi.org/10.1007/s10482-017-0872-0 |
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