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Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide

The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, sub...

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Autores principales: Zamlynska, Katarzyna, Komaniecka, Iwona, Zebracki, Kamil, Mazur, Andrzej, Sroka-Bartnicka, Anna, Choma, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644692/
https://www.ncbi.nlm.nih.gov/pubmed/28409238
http://dx.doi.org/10.1007/s10482-017-0872-0
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author Zamlynska, Katarzyna
Komaniecka, Iwona
Zebracki, Kamil
Mazur, Andrzej
Sroka-Bartnicka, Anna
Choma, Adam
author_facet Zamlynska, Katarzyna
Komaniecka, Iwona
Zebracki, Kamil
Mazur, Andrzej
Sroka-Bartnicka, Anna
Choma, Adam
author_sort Zamlynska, Katarzyna
collection PubMed
description The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes.
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spelling pubmed-56446922017-10-27 Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide Zamlynska, Katarzyna Komaniecka, Iwona Zebracki, Kamil Mazur, Andrzej Sroka-Bartnicka, Anna Choma, Adam Antonie Van Leeuwenhoek Original Paper The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes. Springer International Publishing 2017-04-13 2017 /pmc/articles/PMC5644692/ /pubmed/28409238 http://dx.doi.org/10.1007/s10482-017-0872-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Zamlynska, Katarzyna
Komaniecka, Iwona
Zebracki, Kamil
Mazur, Andrzej
Sroka-Bartnicka, Anna
Choma, Adam
Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title_full Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title_fullStr Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title_full_unstemmed Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title_short Studies on lipid A isolated from Phyllobacterium trifolii PETP02(T) lipopolysaccharide
title_sort studies on lipid a isolated from phyllobacterium trifolii petp02(t) lipopolysaccharide
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5644692/
https://www.ncbi.nlm.nih.gov/pubmed/28409238
http://dx.doi.org/10.1007/s10482-017-0872-0
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