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The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation

We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has empha...

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Autores principales: Golkaram, Mahdi, Jang, Jiwon, Hellander, Stefan, Kosik, Kenneth S., Petzold, Linda R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645312/
https://www.ncbi.nlm.nih.gov/pubmed/29042584
http://dx.doi.org/10.1038/s41598-017-13731-3
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author Golkaram, Mahdi
Jang, Jiwon
Hellander, Stefan
Kosik, Kenneth S.
Petzold, Linda R.
author_facet Golkaram, Mahdi
Jang, Jiwon
Hellander, Stefan
Kosik, Kenneth S.
Petzold, Linda R.
author_sort Golkaram, Mahdi
collection PubMed
description We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells.
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spelling pubmed-56453122017-10-26 The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation Golkaram, Mahdi Jang, Jiwon Hellander, Stefan Kosik, Kenneth S. Petzold, Linda R. Sci Rep Article We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells. Nature Publishing Group UK 2017-10-17 /pmc/articles/PMC5645312/ /pubmed/29042584 http://dx.doi.org/10.1038/s41598-017-13731-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Golkaram, Mahdi
Jang, Jiwon
Hellander, Stefan
Kosik, Kenneth S.
Petzold, Linda R.
The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title_full The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title_fullStr The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title_full_unstemmed The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title_short The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
title_sort role of chromatin density in cell population heterogeneity during stem cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645312/
https://www.ncbi.nlm.nih.gov/pubmed/29042584
http://dx.doi.org/10.1038/s41598-017-13731-3
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