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The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation
We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has empha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645312/ https://www.ncbi.nlm.nih.gov/pubmed/29042584 http://dx.doi.org/10.1038/s41598-017-13731-3 |
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author | Golkaram, Mahdi Jang, Jiwon Hellander, Stefan Kosik, Kenneth S. Petzold, Linda R. |
author_facet | Golkaram, Mahdi Jang, Jiwon Hellander, Stefan Kosik, Kenneth S. Petzold, Linda R. |
author_sort | Golkaram, Mahdi |
collection | PubMed |
description | We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells. |
format | Online Article Text |
id | pubmed-5645312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56453122017-10-26 The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation Golkaram, Mahdi Jang, Jiwon Hellander, Stefan Kosik, Kenneth S. Petzold, Linda R. Sci Rep Article We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells. Nature Publishing Group UK 2017-10-17 /pmc/articles/PMC5645312/ /pubmed/29042584 http://dx.doi.org/10.1038/s41598-017-13731-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Golkaram, Mahdi Jang, Jiwon Hellander, Stefan Kosik, Kenneth S. Petzold, Linda R. The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title_full | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title_fullStr | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title_full_unstemmed | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title_short | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation |
title_sort | role of chromatin density in cell population heterogeneity during stem cell differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645312/ https://www.ncbi.nlm.nih.gov/pubmed/29042584 http://dx.doi.org/10.1038/s41598-017-13731-3 |
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