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Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers

The extracellular matrix (ECM) is a fibrillar protein-based network, the physical and chemical properties of which can influence a multitude of cellular processes. Despite having an important role in cell and tissue signaling, a complete chemo-mechanical characterization of ECM proteins such as fibr...

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Autores principales: Szymanski, John M., Zhang, Kairui, Feinberg, Adam W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645409/
https://www.ncbi.nlm.nih.gov/pubmed/29042643
http://dx.doi.org/10.1038/s41598-017-13866-3
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author Szymanski, John M.
Zhang, Kairui
Feinberg, Adam W.
author_facet Szymanski, John M.
Zhang, Kairui
Feinberg, Adam W.
author_sort Szymanski, John M.
collection PubMed
description The extracellular matrix (ECM) is a fibrillar protein-based network, the physical and chemical properties of which can influence a multitude of cellular processes. Despite having an important role in cell and tissue signaling, a complete chemo-mechanical characterization of ECM proteins such as fibronectin (FN) is lacking. In this study, we engineered monodisperse FN nanofibers using a surface-initiated assembly technique in order to provide new insight into the elastic behavior of this material over large deformations. FN nanofibers were patterned on surfaces in a pre-stressed state and when released from the surface underwent rapid contraction. We found that the FN nanofibers underwent 3.3-fold and 9-fold changes in length and width, respectively, and that the nanofiber volume was conserved. Volume was also conserved following uniaxial extension of the FN nanofibers of ~2-fold relative to the patterned state. This data suggests that the FN networks we engineered formed an incompressible material with a Poisson’s ratio of ~0.5. While the Poisson’s ratio of cells and other biological materials are widely estimated as 0.5, our experimental results demonstrate that for FN networks this is a reasonable approximation.
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spelling pubmed-56454092017-10-26 Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers Szymanski, John M. Zhang, Kairui Feinberg, Adam W. Sci Rep Article The extracellular matrix (ECM) is a fibrillar protein-based network, the physical and chemical properties of which can influence a multitude of cellular processes. Despite having an important role in cell and tissue signaling, a complete chemo-mechanical characterization of ECM proteins such as fibronectin (FN) is lacking. In this study, we engineered monodisperse FN nanofibers using a surface-initiated assembly technique in order to provide new insight into the elastic behavior of this material over large deformations. FN nanofibers were patterned on surfaces in a pre-stressed state and when released from the surface underwent rapid contraction. We found that the FN nanofibers underwent 3.3-fold and 9-fold changes in length and width, respectively, and that the nanofiber volume was conserved. Volume was also conserved following uniaxial extension of the FN nanofibers of ~2-fold relative to the patterned state. This data suggests that the FN networks we engineered formed an incompressible material with a Poisson’s ratio of ~0.5. While the Poisson’s ratio of cells and other biological materials are widely estimated as 0.5, our experimental results demonstrate that for FN networks this is a reasonable approximation. Nature Publishing Group UK 2017-10-17 /pmc/articles/PMC5645409/ /pubmed/29042643 http://dx.doi.org/10.1038/s41598-017-13866-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Szymanski, John M.
Zhang, Kairui
Feinberg, Adam W.
Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title_full Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title_fullStr Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title_full_unstemmed Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title_short Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers
title_sort measuring the poisson’s ratio of fibronectin using engineered nanofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645409/
https://www.ncbi.nlm.nih.gov/pubmed/29042643
http://dx.doi.org/10.1038/s41598-017-13866-3
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