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Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury
Ischemic stroke is one of the leading causes of adult disability and death. Hyperglycemia is associated with an increased risk of stroke and poor outcomes after brain injury. Dynamin-like protein I (DLP-1) regulates mitochondrial fission and promotes mitochondrial dynamics. Neurodegenerative disease...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association for Laboratory Animal Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645597/ https://www.ncbi.nlm.nih.gov/pubmed/29046694 http://dx.doi.org/10.5625/lar.2017.33.3.202 |
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author | Park, Dong-Ju Kim, Myeong-Ok Koh, Phil-Ok |
author_facet | Park, Dong-Ju Kim, Myeong-Ok Koh, Phil-Ok |
author_sort | Park, Dong-Ju |
collection | PubMed |
description | Ischemic stroke is one of the leading causes of adult disability and death. Hyperglycemia is associated with an increased risk of stroke and poor outcomes after brain injury. Dynamin-like protein I (DLP-1) regulates mitochondrial fission and promotes mitochondrial dynamics. Neurodegenerative diseases are associated with mitochondrial dysfunction, and the downregulation of DLP-1 has been previously identified in a stroke animal model. Here, we investigated the changes in DLP-1 protein expression in an animal model of focal cerebral ischemia with induced hyperglycemia. Streptozotocin (40 mg/kg) was intraperitoneally injected into male rats to induce hyperglycemia, and middle cerebral artery occlusion (MCAO) was surgically induced 4 weeks after streptozotocin treatment. Brain tissue was isolated 24 hours after MCAO, and cerebral cortex samples were used for this study. Proteomics revealed a decrease in DLP-1 expression in MCAO animals when compared with controls, and this downregulation was more prominent in MCAO animals with hyperglycemia. Reverse-transcription polymerase chain reaction and Western blot analyses confirmed that DLP-1 was significantly downregulated in MCAO-injured animals with hyperglycemia compared to those without hyperglycemia. The decrease in DLP-1 indicates mitochondrial morphological changes and dysfunction. Together, these results suggest that the severe decrease of DLP-1 seen after brain injury under hyperglycemic conditions may exacerbate the damage to the brain. |
format | Online Article Text |
id | pubmed-5645597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56455972017-10-18 Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury Park, Dong-Ju Kim, Myeong-Ok Koh, Phil-Ok Lab Anim Res Original Article Ischemic stroke is one of the leading causes of adult disability and death. Hyperglycemia is associated with an increased risk of stroke and poor outcomes after brain injury. Dynamin-like protein I (DLP-1) regulates mitochondrial fission and promotes mitochondrial dynamics. Neurodegenerative diseases are associated with mitochondrial dysfunction, and the downregulation of DLP-1 has been previously identified in a stroke animal model. Here, we investigated the changes in DLP-1 protein expression in an animal model of focal cerebral ischemia with induced hyperglycemia. Streptozotocin (40 mg/kg) was intraperitoneally injected into male rats to induce hyperglycemia, and middle cerebral artery occlusion (MCAO) was surgically induced 4 weeks after streptozotocin treatment. Brain tissue was isolated 24 hours after MCAO, and cerebral cortex samples were used for this study. Proteomics revealed a decrease in DLP-1 expression in MCAO animals when compared with controls, and this downregulation was more prominent in MCAO animals with hyperglycemia. Reverse-transcription polymerase chain reaction and Western blot analyses confirmed that DLP-1 was significantly downregulated in MCAO-injured animals with hyperglycemia compared to those without hyperglycemia. The decrease in DLP-1 indicates mitochondrial morphological changes and dysfunction. Together, these results suggest that the severe decrease of DLP-1 seen after brain injury under hyperglycemic conditions may exacerbate the damage to the brain. Korean Association for Laboratory Animal Science 2017-09 2017-09-27 /pmc/articles/PMC5645597/ /pubmed/29046694 http://dx.doi.org/10.5625/lar.2017.33.3.202 Text en Copyright © 2017 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Dong-Ju Kim, Myeong-Ok Koh, Phil-Ok Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title | Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title_full | Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title_fullStr | Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title_full_unstemmed | Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title_short | Hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
title_sort | hyperglycemia exacerbates downregulation of dynamin-like protein 1 in ischemic cerebral injury |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645597/ https://www.ncbi.nlm.nih.gov/pubmed/29046694 http://dx.doi.org/10.5625/lar.2017.33.3.202 |
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