Cargando…

Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution

Mycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, Mycobacterium leprae and M. lepraemurium were...

Descripción completa

Detalles Bibliográficos
Autores principales: Benjak, Andrej, Honap, Tanvi P., Avanzi, Charlotte, Becerril-Villanueva, Enrique, Estrada-García, Iris, Rojas-Espinosa, Oscar, Stone, Anne C., Cole, Stewart T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646247/
https://www.ncbi.nlm.nih.gov/pubmed/29042494
http://dx.doi.org/10.1128/mBio.01283-17
_version_ 1783272046407450624
author Benjak, Andrej
Honap, Tanvi P.
Avanzi, Charlotte
Becerril-Villanueva, Enrique
Estrada-García, Iris
Rojas-Espinosa, Oscar
Stone, Anne C.
Cole, Stewart T.
author_facet Benjak, Andrej
Honap, Tanvi P.
Avanzi, Charlotte
Becerril-Villanueva, Enrique
Estrada-García, Iris
Rojas-Espinosa, Oscar
Stone, Anne C.
Cole, Stewart T.
author_sort Benjak, Andrej
collection PubMed
description Mycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, Mycobacterium leprae and M. lepraemurium were once thought to be closely related, although it was later suggested that M. lepraemurium might be related to Mycobacterium avium. In this study, the complete genome of M. lepraemurium was sequenced using a combination of PacBio and Illumina sequencing. Phylogenomic analyses confirmed that M. lepraemurium is a distinct species within the M. avium complex (MAC). The M. lepraemurium genome is 4.05 Mb in length, which is considerably smaller than other MAC genomes, and it comprises 2,682 functional genes and 1,139 pseudogenes, which indicates that M. lepraemurium has undergone genome reduction. An error-prone repair homologue of the DNA polymerase III α-subunit was found to be nonfunctional in M. lepraemurium, which might contribute to pseudogene formation due to the accumulation of mutations in nonessential genes. M. lepraemurium has retained the functionality of several genes thought to influence virulence among members of the MAC.
format Online
Article
Text
id pubmed-5646247
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-56462472017-10-23 Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution Benjak, Andrej Honap, Tanvi P. Avanzi, Charlotte Becerril-Villanueva, Enrique Estrada-García, Iris Rojas-Espinosa, Oscar Stone, Anne C. Cole, Stewart T. mBio Observation Mycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, Mycobacterium leprae and M. lepraemurium were once thought to be closely related, although it was later suggested that M. lepraemurium might be related to Mycobacterium avium. In this study, the complete genome of M. lepraemurium was sequenced using a combination of PacBio and Illumina sequencing. Phylogenomic analyses confirmed that M. lepraemurium is a distinct species within the M. avium complex (MAC). The M. lepraemurium genome is 4.05 Mb in length, which is considerably smaller than other MAC genomes, and it comprises 2,682 functional genes and 1,139 pseudogenes, which indicates that M. lepraemurium has undergone genome reduction. An error-prone repair homologue of the DNA polymerase III α-subunit was found to be nonfunctional in M. lepraemurium, which might contribute to pseudogene formation due to the accumulation of mutations in nonessential genes. M. lepraemurium has retained the functionality of several genes thought to influence virulence among members of the MAC. American Society for Microbiology 2017-10-17 /pmc/articles/PMC5646247/ /pubmed/29042494 http://dx.doi.org/10.1128/mBio.01283-17 Text en Copyright © 2017 Benjak et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Observation
Benjak, Andrej
Honap, Tanvi P.
Avanzi, Charlotte
Becerril-Villanueva, Enrique
Estrada-García, Iris
Rojas-Espinosa, Oscar
Stone, Anne C.
Cole, Stewart T.
Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title_full Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title_fullStr Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title_full_unstemmed Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title_short Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
title_sort insights from the genome sequence of mycobacterium lepraemurium: massive gene decay and reductive evolution
topic Observation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646247/
https://www.ncbi.nlm.nih.gov/pubmed/29042494
http://dx.doi.org/10.1128/mBio.01283-17
work_keys_str_mv AT benjakandrej insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT honaptanvip insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT avanzicharlotte insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT becerrilvillanuevaenrique insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT estradagarciairis insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT rojasespinosaoscar insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT stoneannec insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution
AT colestewartt insightsfromthegenomesequenceofmycobacteriumlepraemuriummassivegenedecayandreductiveevolution