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Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation

In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma c...

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Autores principales: Zhu, Xinyao, Zhang, Nan, Wang, Zuobin, Liu, X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646263/
https://www.ncbi.nlm.nih.gov/pubmed/29082126
http://dx.doi.org/10.1007/s12213-016-0089-8
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author Zhu, Xinyao
Zhang, Nan
Wang, Zuobin
Liu, X.
author_facet Zhu, Xinyao
Zhang, Nan
Wang, Zuobin
Liu, X.
author_sort Zhu, Xinyao
collection PubMed
description In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young’s modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment.
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spelling pubmed-56462632017-10-27 Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation Zhu, Xinyao Zhang, Nan Wang, Zuobin Liu, X. J Microbio Robot Research Paper In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young’s modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment. Springer Berlin Heidelberg 2016-05-07 2016 /pmc/articles/PMC5646263/ /pubmed/29082126 http://dx.doi.org/10.1007/s12213-016-0089-8 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Paper
Zhu, Xinyao
Zhang, Nan
Wang, Zuobin
Liu, X.
Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title_full Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title_fullStr Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title_full_unstemmed Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title_short Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
title_sort investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by afm nanoindentation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646263/
https://www.ncbi.nlm.nih.gov/pubmed/29082126
http://dx.doi.org/10.1007/s12213-016-0089-8
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