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The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts
There is increasing interest in the possible role of the oral microbiome in oral squamous cell carcinoma (OSSC). However, studies on the possible association between the oral bacteriome and OSCC remain inconclusive, while the potential role of the “mycobiome” in oral carcinogenesis has never been ex...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646595/ http://dx.doi.org/10.1080/20002297.2017.1325188 |
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author | Al-hebshi, Nezar Perera, Manosha Perera, Irosha Ipe, Deepak Ulett, Glen Speicher, David Nasher, Akram Maryoud, Mohamed Homeida, Husham Idris, Ali Mohamed Chen, Tsute Johnson, Newell |
author_facet | Al-hebshi, Nezar Perera, Manosha Perera, Irosha Ipe, Deepak Ulett, Glen Speicher, David Nasher, Akram Maryoud, Mohamed Homeida, Husham Idris, Ali Mohamed Chen, Tsute Johnson, Newell |
author_sort | Al-hebshi, Nezar |
collection | PubMed |
description | There is increasing interest in the possible role of the oral microbiome in oral squamous cell carcinoma (OSSC). However, studies on the possible association between the oral bacteriome and OSCC remain inconclusive, while the potential role of the “mycobiome” in oral carcinogenesis has never been explored. I hereby present results from our recent studies on the bacteriome and mycobiome associated with OSCC based on analysis of samples from Yemeni and Sri Lankan cohorts. Tissue biopsies were obtained from the cases while deep buccal swabs or fibro-epithelial polyps were collected from the controls. Sequencing of the bacterial V1-V3 region of the 16S rRNA gene and the fungal ITS2 region using Illumina’s 2x300 bp chemistry was employed to study the bacteriome and mycobiome, respectively. Merged reads were classified to species level using a BLASTN-algorithm. Downstream analyses were performed using QIIME, PICRUSt, and LEfSe. Fusobacterium nucleatum subsp. polymorphum, Pseudomonas aeruginosa, Prevotella and Campylobacter spp. were more abundant in OSCC samples while Streptococcus mitis and Rothia spp. were overrepresented in the controls. Functionally, inflammatory bacterial attributes including bacterial mobility, flagellar assembly, bacterial chemotaxis and LPS synthesis were enriched in the tumors. Mycobiome analysis revealed a dysbiotic fungal community dominated by C. albicans in association with OSCC. |
format | Online Article Text |
id | pubmed-5646595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-56465952017-10-27 The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts Al-hebshi, Nezar Perera, Manosha Perera, Irosha Ipe, Deepak Ulett, Glen Speicher, David Nasher, Akram Maryoud, Mohamed Homeida, Husham Idris, Ali Mohamed Chen, Tsute Johnson, Newell J Oral Microbiol Session-1: Oral microbial ecology and the oral microbiome There is increasing interest in the possible role of the oral microbiome in oral squamous cell carcinoma (OSSC). However, studies on the possible association between the oral bacteriome and OSCC remain inconclusive, while the potential role of the “mycobiome” in oral carcinogenesis has never been explored. I hereby present results from our recent studies on the bacteriome and mycobiome associated with OSCC based on analysis of samples from Yemeni and Sri Lankan cohorts. Tissue biopsies were obtained from the cases while deep buccal swabs or fibro-epithelial polyps were collected from the controls. Sequencing of the bacterial V1-V3 region of the 16S rRNA gene and the fungal ITS2 region using Illumina’s 2x300 bp chemistry was employed to study the bacteriome and mycobiome, respectively. Merged reads were classified to species level using a BLASTN-algorithm. Downstream analyses were performed using QIIME, PICRUSt, and LEfSe. Fusobacterium nucleatum subsp. polymorphum, Pseudomonas aeruginosa, Prevotella and Campylobacter spp. were more abundant in OSCC samples while Streptococcus mitis and Rothia spp. were overrepresented in the controls. Functionally, inflammatory bacterial attributes including bacterial mobility, flagellar assembly, bacterial chemotaxis and LPS synthesis were enriched in the tumors. Mycobiome analysis revealed a dysbiotic fungal community dominated by C. albicans in association with OSCC. Taylor & Francis 2017-06-09 /pmc/articles/PMC5646595/ http://dx.doi.org/10.1080/20002297.2017.1325188 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Session-1: Oral microbial ecology and the oral microbiome Al-hebshi, Nezar Perera, Manosha Perera, Irosha Ipe, Deepak Ulett, Glen Speicher, David Nasher, Akram Maryoud, Mohamed Homeida, Husham Idris, Ali Mohamed Chen, Tsute Johnson, Newell The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title | The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title_full | The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title_fullStr | The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title_full_unstemmed | The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title_short | The bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from Yemeni and Sri Lankan cohorts |
title_sort | bacteriome and mycobiome associated with oral squamous cell carcinoma: metagenomic analysis of samples from yemeni and sri lankan cohorts |
topic | Session-1: Oral microbial ecology and the oral microbiome |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646595/ http://dx.doi.org/10.1080/20002297.2017.1325188 |
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