Shotgun proteomic analysis of Anaeroglobus geminatus

Objectives: Anaeroglobus geminatus is a gram-negative species belonging to the family of Veillonellaceae and close associate to genus Megasphaera. The role of the species within dysbiotic microbial communities is not yet clear. However, this putative pathogen has been identified in a number of anaerobic infections, including periodontal diseases. The aim of this proteomic study was to analyse the global protein expression of A. geminatus. Methods: The whole proteome of A. geminatus CCUG 44773 strain was analysed by shotgun proteomics, using Orbitrap Fusion mass spectrometer. Results: A total of 1164 A. geminatus proteins were identified by this analysis, with an FDR of 2.2 % and 0.19 %, at peptide and protein level, respectively. Functional analysis of the identified proteins was performed using the Uniprot knowledge-base. Among those, 100 proteins were predicated to be transferase, 47 were hydrolases, and 41 were ligases, based on computational analysed functions. The top-scored pathways were “amino-acid biosynthesis” and “cofactor biosynthesis”, and each of these pathways included 20 proteins, such as arginine repressor and aspartokinase. Conclusion: According to our knowledge, this is the first study that gives an overview of the global proteome of A. geminatus and hopefully will help to unravel its role in anaerobic infections.