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Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci

Streptococcus species are the most abundant genera in the oral microbiota. The prevalence of antibiotic resistant oral streptococci is on the increase and the most reported antibiotic resistance profile in oral streptococci include, among several antibiotics, resistance to β-lactams antibiotics, mac...

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Autor principal: Lunde, Tracy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646629/
http://dx.doi.org/10.1080/20002297.2017.1325273
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author Lunde, Tracy
author_facet Lunde, Tracy
author_sort Lunde, Tracy
collection PubMed
description Streptococcus species are the most abundant genera in the oral microbiota. The prevalence of antibiotic resistant oral streptococci is on the increase and the most reported antibiotic resistance profile in oral streptococci include, among several antibiotics, resistance to β-lactams antibiotics, macrolides and tetracycline. Several studies have shown that majority of the antibiotic resistance genes that have been isolated in oral streptococci are usually associated with mobile genetic elements such as plasmids and conjugative transposons. In the current study, we used molecular based techniques to screen a total of 100 clinical oral streptococci strains isolated in Norway for the presence of Tn916/Tn916-like elements. We also investigated the diversity of these elements in oral streptococci by Restriction Fragment Length Polymorphism (RFLP) and DNA sequencing. It was found that a total of 34 strains were positive for tetracycline resistance gene, tet(M), and integrase gene (int) belonging to the Tn916-like family. The minimum inhibitory concentration (MIC) of these tetracycline-resistant oral streptococci ranged between 2-64 µg/mL. Most of the positive strains for tet(M) and int genes harbor the wild type Tn916 and a few harbor other Tn916-like elements suggesting high versatility of the Tn916 element in oral streptococci in Norway.
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spelling pubmed-56466292017-10-27 Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci Lunde, Tracy J Oral Microbiol Poster Session Streptococcus species are the most abundant genera in the oral microbiota. The prevalence of antibiotic resistant oral streptococci is on the increase and the most reported antibiotic resistance profile in oral streptococci include, among several antibiotics, resistance to β-lactams antibiotics, macrolides and tetracycline. Several studies have shown that majority of the antibiotic resistance genes that have been isolated in oral streptococci are usually associated with mobile genetic elements such as plasmids and conjugative transposons. In the current study, we used molecular based techniques to screen a total of 100 clinical oral streptococci strains isolated in Norway for the presence of Tn916/Tn916-like elements. We also investigated the diversity of these elements in oral streptococci by Restriction Fragment Length Polymorphism (RFLP) and DNA sequencing. It was found that a total of 34 strains were positive for tetracycline resistance gene, tet(M), and integrase gene (int) belonging to the Tn916-like family. The minimum inhibitory concentration (MIC) of these tetracycline-resistant oral streptococci ranged between 2-64 µg/mL. Most of the positive strains for tet(M) and int genes harbor the wild type Tn916 and a few harbor other Tn916-like elements suggesting high versatility of the Tn916 element in oral streptococci in Norway. Taylor & Francis 2017-06-12 /pmc/articles/PMC5646629/ http://dx.doi.org/10.1080/20002297.2017.1325273 Text en Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Poster Session
Lunde, Tracy
Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title_full Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title_fullStr Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title_full_unstemmed Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title_short Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci
title_sort investigation of the prevalence of tn916/tn916-like elements in oral streptococci
topic Poster Session
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646629/
http://dx.doi.org/10.1080/20002297.2017.1325273
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