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Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria
The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based P...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646718/ https://www.ncbi.nlm.nih.gov/pubmed/29071186 http://dx.doi.org/10.7150/ntno.18720 |
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author | Kim, Jinjoo Kim, Hansol Park, Ji Ho Jon, Sangyong |
author_facet | Kim, Jinjoo Kim, Hansol Park, Ji Ho Jon, Sangyong |
author_sort | Kim, Jinjoo |
collection | PubMed |
description | The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In the photo-PCR system, poly(enthylene glycol)-modified gold nanorods (PEG-GNRs), used as a heat generator, are added into the PCR mixture, which is subsequently periodically irradiated with a 808-nm laser to create thermal cycling. Photo-PCR was able to significantly reduce overall thermal cycling time by integrating bacterial cell lysis and DNA amplification into a single step. Furthermore, when combined with KAPA2G fast polymerase and cooling system, the entire process of bacterial genomic DNA extraction and amplification was further shortened, highlighting the potential of photo-PCR for use in a portable, POC diagnostic system. |
format | Online Article Text |
id | pubmed-5646718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-56467182017-10-25 Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria Kim, Jinjoo Kim, Hansol Park, Ji Ho Jon, Sangyong Nanotheranostics Research Paper The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In the photo-PCR system, poly(enthylene glycol)-modified gold nanorods (PEG-GNRs), used as a heat generator, are added into the PCR mixture, which is subsequently periodically irradiated with a 808-nm laser to create thermal cycling. Photo-PCR was able to significantly reduce overall thermal cycling time by integrating bacterial cell lysis and DNA amplification into a single step. Furthermore, when combined with KAPA2G fast polymerase and cooling system, the entire process of bacterial genomic DNA extraction and amplification was further shortened, highlighting the potential of photo-PCR for use in a portable, POC diagnostic system. Ivyspring International Publisher 2017-05-06 /pmc/articles/PMC5646718/ /pubmed/29071186 http://dx.doi.org/10.7150/ntno.18720 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Kim, Jinjoo Kim, Hansol Park, Ji Ho Jon, Sangyong Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title | Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title_full | Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title_fullStr | Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title_full_unstemmed | Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title_short | Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria |
title_sort | gold nanorod-based photo-pcr system for one-step, rapid detection of bacteria |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646718/ https://www.ncbi.nlm.nih.gov/pubmed/29071186 http://dx.doi.org/10.7150/ntno.18720 |
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