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The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae he...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646765/ https://www.ncbi.nlm.nih.gov/pubmed/29045428 http://dx.doi.org/10.1371/journal.pone.0185416 |
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author | Barraza, Carla E. Solari, Clara A. Marcovich, Irina Kershaw, Christopher Galello, Fiorella Rossi, Silvia Ashe, Mark P. Portela, Paula |
author_facet | Barraza, Carla E. Solari, Clara A. Marcovich, Irina Kershaw, Christopher Galello, Fiorella Rossi, Silvia Ashe, Mark P. Portela, Paula |
author_sort | Barraza, Carla E. |
collection | PubMed |
description | Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex. Deletion of TPK2 or TPK3 impacts upon the translational response to heat stress of several mRNAs including CYC1, HSP42, HSP30 and ENO2. TPK2 deletion leads to a robust translational arrest, an increase in SGs/PBs aggregation and translational hypersensitivity to heat stress, whereas TPK3 deletion represses SGs/PBs formation, translational arrest and response for the analyzed mRNAs. Therefore, this work provides evidence indicating that Tpk2 and Tpk3 have opposing roles in translational adaptation during heat stress, and highlight how the same signaling pathway can be regulated to generate strikingly distinct physiological outputs. |
format | Online Article Text |
id | pubmed-5646765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56467652017-10-30 The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae Barraza, Carla E. Solari, Clara A. Marcovich, Irina Kershaw, Christopher Galello, Fiorella Rossi, Silvia Ashe, Mark P. Portela, Paula PLoS One Research Article Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex. Deletion of TPK2 or TPK3 impacts upon the translational response to heat stress of several mRNAs including CYC1, HSP42, HSP30 and ENO2. TPK2 deletion leads to a robust translational arrest, an increase in SGs/PBs aggregation and translational hypersensitivity to heat stress, whereas TPK3 deletion represses SGs/PBs formation, translational arrest and response for the analyzed mRNAs. Therefore, this work provides evidence indicating that Tpk2 and Tpk3 have opposing roles in translational adaptation during heat stress, and highlight how the same signaling pathway can be regulated to generate strikingly distinct physiological outputs. Public Library of Science 2017-10-18 /pmc/articles/PMC5646765/ /pubmed/29045428 http://dx.doi.org/10.1371/journal.pone.0185416 Text en © 2017 Barraza et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barraza, Carla E. Solari, Clara A. Marcovich, Irina Kershaw, Christopher Galello, Fiorella Rossi, Silvia Ashe, Mark P. Portela, Paula The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title | The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title_full | The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title_fullStr | The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title_full_unstemmed | The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title_short | The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae |
title_sort | role of pka in the translational response to heat stress in saccharomyces cerevisiae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646765/ https://www.ncbi.nlm.nih.gov/pubmed/29045428 http://dx.doi.org/10.1371/journal.pone.0185416 |
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