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Grb2-associated binder 2 expression and its roles in uveal melanoma invasion
Uveal melanoma (UM) is characterized by high metastasis and poor prognosis. A more improved understanding of the metastatic mechanism in UM cells is essential for the design of molecular therapy. Grb2-associated binder 2 (Gab2) has been reported to serve important roles in the progression of various...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646995/ https://www.ncbi.nlm.nih.gov/pubmed/28791340 http://dx.doi.org/10.3892/mmr.2017.7151 |
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author | Chen, Meiling Li, Yuehua Sun, Xiuning Zhang, Baogang Li, Wei Wang, Shuxiao Zhu, Xuetao Li, Feng Shi, Lihong |
author_facet | Chen, Meiling Li, Yuehua Sun, Xiuning Zhang, Baogang Li, Wei Wang, Shuxiao Zhu, Xuetao Li, Feng Shi, Lihong |
author_sort | Chen, Meiling |
collection | PubMed |
description | Uveal melanoma (UM) is characterized by high metastasis and poor prognosis. A more improved understanding of the metastatic mechanism in UM cells is essential for the design of molecular therapy. Grb2-associated binder 2 (Gab2) has been reported to serve important roles in the progression of various types of human cancer. However, the role of Gab2 in the migration and invasion of UM remains unclear. The present study sought to further assess the expression of Gab2 in UM and the role of Gab2 in the invasion of UM cells. Clinical UM tissue samples and UM cell lines were analyzed using western blot analysis for the expression of Gab2. RNA interference was used to investigate the effect of Gab2 on the migratory and invasive characteristics of UM cells in vitro. The expression levels of matrix metalloproteinase (MMP)2, MMP9 and fascin in Gab2-knockdown, and control cells were also detected using western blot analysis. A total of 20 clinical UM samples and a subset of UM cell lines were investigated with uniformly high Gab2 expression. In the in vitro experiment, reduction of Gab2 using small interfering RNA inhibited the migration and invasion of UM cells by mediating MMPs, and fascin expression. These data suggest that Gab2 is a useful prognostic marker for UM and a novel therapeutic target for UM metastasis intervention. |
format | Online Article Text |
id | pubmed-5646995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56469952017-10-24 Grb2-associated binder 2 expression and its roles in uveal melanoma invasion Chen, Meiling Li, Yuehua Sun, Xiuning Zhang, Baogang Li, Wei Wang, Shuxiao Zhu, Xuetao Li, Feng Shi, Lihong Mol Med Rep Articles Uveal melanoma (UM) is characterized by high metastasis and poor prognosis. A more improved understanding of the metastatic mechanism in UM cells is essential for the design of molecular therapy. Grb2-associated binder 2 (Gab2) has been reported to serve important roles in the progression of various types of human cancer. However, the role of Gab2 in the migration and invasion of UM remains unclear. The present study sought to further assess the expression of Gab2 in UM and the role of Gab2 in the invasion of UM cells. Clinical UM tissue samples and UM cell lines were analyzed using western blot analysis for the expression of Gab2. RNA interference was used to investigate the effect of Gab2 on the migratory and invasive characteristics of UM cells in vitro. The expression levels of matrix metalloproteinase (MMP)2, MMP9 and fascin in Gab2-knockdown, and control cells were also detected using western blot analysis. A total of 20 clinical UM samples and a subset of UM cell lines were investigated with uniformly high Gab2 expression. In the in vitro experiment, reduction of Gab2 using small interfering RNA inhibited the migration and invasion of UM cells by mediating MMPs, and fascin expression. These data suggest that Gab2 is a useful prognostic marker for UM and a novel therapeutic target for UM metastasis intervention. D.A. Spandidos 2017-10 2017-08-03 /pmc/articles/PMC5646995/ /pubmed/28791340 http://dx.doi.org/10.3892/mmr.2017.7151 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Chen, Meiling Li, Yuehua Sun, Xiuning Zhang, Baogang Li, Wei Wang, Shuxiao Zhu, Xuetao Li, Feng Shi, Lihong Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title | Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title_full | Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title_fullStr | Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title_full_unstemmed | Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title_short | Grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
title_sort | grb2-associated binder 2 expression and its roles in uveal melanoma invasion |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646995/ https://www.ncbi.nlm.nih.gov/pubmed/28791340 http://dx.doi.org/10.3892/mmr.2017.7151 |
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