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Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells

Zinc (Zn) deficiency is important for inducing nucleotide-binding domain and leucine-rich repeat-containing family, pyrin domain-containing-3 (NLRP3) inflammasome activation in macrophages. However, its function in the NLRP3 inflammasome activation of peritoneal mesothelial cells (PMCs) remains to b...

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Autores principales: Fan, Yi, Zhang, Xiuli, Yang, Lina, Wang, Jun, Hu, Ye, Bian, Aishu, Liu, Jin, Ma, Jianfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647055/
https://www.ncbi.nlm.nih.gov/pubmed/28849014
http://dx.doi.org/10.3892/mmr.2017.7236
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author Fan, Yi
Zhang, Xiuli
Yang, Lina
Wang, Jun
Hu, Ye
Bian, Aishu
Liu, Jin
Ma, Jianfei
author_facet Fan, Yi
Zhang, Xiuli
Yang, Lina
Wang, Jun
Hu, Ye
Bian, Aishu
Liu, Jin
Ma, Jianfei
author_sort Fan, Yi
collection PubMed
description Zinc (Zn) deficiency is important for inducing nucleotide-binding domain and leucine-rich repeat-containing family, pyrin domain-containing-3 (NLRP3) inflammasome activation in macrophages. However, its function in the NLRP3 inflammasome activation of peritoneal mesothelial cells (PMCs) remains to be elucidated. In the present study, the human PMC (HPMC) line HMrSV5 was co-treated with high glucose and either ZnSO4 or a Zn chelator. The activity of the NLRP3/caspase-1 inflammasome was assessed via western blot analysis, immunofluorescence, reverse transcription-quantitative polymerase chain reaction and ELISA. In addition, the activity of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was detected using western blotting, and the level of reactive oxygen species (ROS) was assessed by 2,7-dichlorofluorescein fluorescence and flow cytometry. It was found that Zn supplementation inhibited HG-induced NLRP3 inflammasome activation in the HPMCs by attenuating ROS production. Further experiments revealed that Zn supplementation inhibited the HG-induced production of ROS through activation of the Nrf2 antioxidant pathway. These results indicated that Zn inhibited NLRP3 inflammasome activation in the HG-treated HPMCs by activating the Nrf2 antioxidant pathway and reducing the production of ROS.
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spelling pubmed-56470552017-10-24 Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells Fan, Yi Zhang, Xiuli Yang, Lina Wang, Jun Hu, Ye Bian, Aishu Liu, Jin Ma, Jianfei Mol Med Rep Articles Zinc (Zn) deficiency is important for inducing nucleotide-binding domain and leucine-rich repeat-containing family, pyrin domain-containing-3 (NLRP3) inflammasome activation in macrophages. However, its function in the NLRP3 inflammasome activation of peritoneal mesothelial cells (PMCs) remains to be elucidated. In the present study, the human PMC (HPMC) line HMrSV5 was co-treated with high glucose and either ZnSO4 or a Zn chelator. The activity of the NLRP3/caspase-1 inflammasome was assessed via western blot analysis, immunofluorescence, reverse transcription-quantitative polymerase chain reaction and ELISA. In addition, the activity of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was detected using western blotting, and the level of reactive oxygen species (ROS) was assessed by 2,7-dichlorofluorescein fluorescence and flow cytometry. It was found that Zn supplementation inhibited HG-induced NLRP3 inflammasome activation in the HPMCs by attenuating ROS production. Further experiments revealed that Zn supplementation inhibited the HG-induced production of ROS through activation of the Nrf2 antioxidant pathway. These results indicated that Zn inhibited NLRP3 inflammasome activation in the HG-treated HPMCs by activating the Nrf2 antioxidant pathway and reducing the production of ROS. D.A. Spandidos 2017-10 2017-08-11 /pmc/articles/PMC5647055/ /pubmed/28849014 http://dx.doi.org/10.3892/mmr.2017.7236 Text en Copyright: © Fan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Fan, Yi
Zhang, Xiuli
Yang, Lina
Wang, Jun
Hu, Ye
Bian, Aishu
Liu, Jin
Ma, Jianfei
Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title_full Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title_fullStr Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title_full_unstemmed Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title_short Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
title_sort zinc inhibits high glucose-induced nlrp3 inflammasome activation in human peritoneal mesothelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647055/
https://www.ncbi.nlm.nih.gov/pubmed/28849014
http://dx.doi.org/10.3892/mmr.2017.7236
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