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Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats

In the current study, the hypothesis that testicular varicocelectomy improves spermatogenesis and attenuates apoptosis via the induction of heat shock protein 70 (Hsp70) in a rat model of varicocele was investigated. Adult male Wistar rats (n=75) were randomly divided into 5 groups of 15 each: Contr...

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Autores principales: Ning, Jin-Zhuo, Rao, Ting, Cheng, Fan, Yu, Wei-Min, Ruan, Yuan, Yuan, Run, Zhu, Shao-Ming, Du, Yang, Xiao, Cheng-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647084/
https://www.ncbi.nlm.nih.gov/pubmed/28849011
http://dx.doi.org/10.3892/mmr.2017.7239
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author Ning, Jin-Zhuo
Rao, Ting
Cheng, Fan
Yu, Wei-Min
Ruan, Yuan
Yuan, Run
Zhu, Shao-Ming
Du, Yang
Xiao, Cheng-Cheng
author_facet Ning, Jin-Zhuo
Rao, Ting
Cheng, Fan
Yu, Wei-Min
Ruan, Yuan
Yuan, Run
Zhu, Shao-Ming
Du, Yang
Xiao, Cheng-Cheng
author_sort Ning, Jin-Zhuo
collection PubMed
description In the current study, the hypothesis that testicular varicocelectomy improves spermatogenesis and attenuates apoptosis via the induction of heat shock protein 70 (Hsp70) in a rat model of varicocele was investigated. Adult male Wistar rats (n=75) were randomly divided into 5 groups of 15 each: Control, sham, varicocele, varicocelectomy, and varicocelectomy plus Quercetin. A total of 6 weeks after the varicocelectomy, the left testis of all rats was removed for subsequent examination. Histological changes were compared between the groups. The expression of Hsp70 and apoptosis-associated indicators were evaluated based on immunohistochemical, western blot and mRNA expression analyses. Compared with the varicocele group, the varicocelectomy group exhibited a markedly reduced Bcl-2-associated X protein/B-cell lymphoma 2 (Bax/Bcl-2) ratio, and had a decreased expression of caspase-9, cytochrome c (cyt c) and caspase-3 through the intrinsic signal transduction pathways. Quercetin treatment inhibited the protective effects of varicocelectomy. The expression of Hsp70 was increased in the varicocele group which was further elevated by the varicocelectomy. These results indicated that varicocelectomy can reduce the Bax/Bcl-2 ratio, and decrease the levels of caspase-9, cyt c and caspase-3 via the mitochondrial signal transduction pathway. Such protective effects on left testis spermatogenesis and against apoptosis may be due to the induction of Hsp70. The findings of the present study suggested that varicocelectomy has a clear advantage in protecting testicular function and ameliorating spermatogenic cells apoptosis.
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spelling pubmed-56470842017-10-24 Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats Ning, Jin-Zhuo Rao, Ting Cheng, Fan Yu, Wei-Min Ruan, Yuan Yuan, Run Zhu, Shao-Ming Du, Yang Xiao, Cheng-Cheng Mol Med Rep Articles In the current study, the hypothesis that testicular varicocelectomy improves spermatogenesis and attenuates apoptosis via the induction of heat shock protein 70 (Hsp70) in a rat model of varicocele was investigated. Adult male Wistar rats (n=75) were randomly divided into 5 groups of 15 each: Control, sham, varicocele, varicocelectomy, and varicocelectomy plus Quercetin. A total of 6 weeks after the varicocelectomy, the left testis of all rats was removed for subsequent examination. Histological changes were compared between the groups. The expression of Hsp70 and apoptosis-associated indicators were evaluated based on immunohistochemical, western blot and mRNA expression analyses. Compared with the varicocele group, the varicocelectomy group exhibited a markedly reduced Bcl-2-associated X protein/B-cell lymphoma 2 (Bax/Bcl-2) ratio, and had a decreased expression of caspase-9, cytochrome c (cyt c) and caspase-3 through the intrinsic signal transduction pathways. Quercetin treatment inhibited the protective effects of varicocelectomy. The expression of Hsp70 was increased in the varicocele group which was further elevated by the varicocelectomy. These results indicated that varicocelectomy can reduce the Bax/Bcl-2 ratio, and decrease the levels of caspase-9, cyt c and caspase-3 via the mitochondrial signal transduction pathway. Such protective effects on left testis spermatogenesis and against apoptosis may be due to the induction of Hsp70. The findings of the present study suggested that varicocelectomy has a clear advantage in protecting testicular function and ameliorating spermatogenic cells apoptosis. D.A. Spandidos 2017-10 2017-08-14 /pmc/articles/PMC5647084/ /pubmed/28849011 http://dx.doi.org/10.3892/mmr.2017.7239 Text en Copyright: © Ning et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ning, Jin-Zhuo
Rao, Ting
Cheng, Fan
Yu, Wei-Min
Ruan, Yuan
Yuan, Run
Zhu, Shao-Ming
Du, Yang
Xiao, Cheng-Cheng
Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title_full Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title_fullStr Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title_full_unstemmed Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title_short Effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
title_sort effect of varicocelectomy treatment on spermatogenesis and apoptosis via the induction of heat shock protein 70 in varicocele-induced rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647084/
https://www.ncbi.nlm.nih.gov/pubmed/28849011
http://dx.doi.org/10.3892/mmr.2017.7239
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