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Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa

The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein an...

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Autores principales: Zheng, Xiangfeng, Yang, Qiya, Zhao, Lina, Apaliya, Maurice Tibiru, Zhang, Xiaoyun, Zhang, Hongyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647337/
https://www.ncbi.nlm.nih.gov/pubmed/29044224
http://dx.doi.org/10.1038/s41598-017-14078-5
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author Zheng, Xiangfeng
Yang, Qiya
Zhao, Lina
Apaliya, Maurice Tibiru
Zhang, Xiaoyun
Zhang, Hongyin
author_facet Zheng, Xiangfeng
Yang, Qiya
Zhao, Lina
Apaliya, Maurice Tibiru
Zhang, Xiaoyun
Zhang, Hongyin
author_sort Zheng, Xiangfeng
collection PubMed
description The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein and glutathione S-transferase involved in detoxification was significantly up-regulated. In addition, the expression levels of zinc-binding oxidoreductase and quinone oxidoreductase that are involved in antioxidant process, ABC transport and MFS transport responsible for chemical transport were activated when treated with patulin. The quantitative real time PCR (qRT-PCR) result also indicated these genes expression levels were increased when treated with patulin. Kac changes accompanying degradation of patulin in R. mucilaginosa were also observed. Totally, 130 Kac sites in 103 proteins were differentially expressed under patulin stress. The differentially up expressed modified proteins were mainly involved in tricarboxylic acid cycle and nuclear acid biosynthesis. The differentially down expressed Kac proteins were mainly classified to ribosome, oxidative phosphorylation, protein synthesis and defense to stress process. Our results suggest that patulin exposure prompt R. mucilaginosa to produce a series of actions to resist or degrade patulin, including Kac. In addition, the Kac information in R. mucilaginosa and Kac in response to patulin stress was firstly revealed.
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spelling pubmed-56473372017-10-26 Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa Zheng, Xiangfeng Yang, Qiya Zhao, Lina Apaliya, Maurice Tibiru Zhang, Xiaoyun Zhang, Hongyin Sci Rep Article The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein and glutathione S-transferase involved in detoxification was significantly up-regulated. In addition, the expression levels of zinc-binding oxidoreductase and quinone oxidoreductase that are involved in antioxidant process, ABC transport and MFS transport responsible for chemical transport were activated when treated with patulin. The quantitative real time PCR (qRT-PCR) result also indicated these genes expression levels were increased when treated with patulin. Kac changes accompanying degradation of patulin in R. mucilaginosa were also observed. Totally, 130 Kac sites in 103 proteins were differentially expressed under patulin stress. The differentially up expressed modified proteins were mainly involved in tricarboxylic acid cycle and nuclear acid biosynthesis. The differentially down expressed Kac proteins were mainly classified to ribosome, oxidative phosphorylation, protein synthesis and defense to stress process. Our results suggest that patulin exposure prompt R. mucilaginosa to produce a series of actions to resist or degrade patulin, including Kac. In addition, the Kac information in R. mucilaginosa and Kac in response to patulin stress was firstly revealed. Nature Publishing Group UK 2017-10-18 /pmc/articles/PMC5647337/ /pubmed/29044224 http://dx.doi.org/10.1038/s41598-017-14078-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zheng, Xiangfeng
Yang, Qiya
Zhao, Lina
Apaliya, Maurice Tibiru
Zhang, Xiaoyun
Zhang, Hongyin
Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title_full Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title_fullStr Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title_full_unstemmed Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title_short Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa
title_sort crosstalk between proteins expression and lysine acetylation in response to patulin stress in rhodotorula mucilaginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647337/
https://www.ncbi.nlm.nih.gov/pubmed/29044224
http://dx.doi.org/10.1038/s41598-017-14078-5
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