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Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis

The diagnostic value of magnetic resonance spectroscopy (MRS), T2-weighted imaging (T2WI) and serum markers of brain injury in a rat model of sepsis were investigated. Rats were randomly divided into the control group and 6, 12 and 24 h after lipopolysaccharide-injection groups. Brain morphology and...

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Autores principales: Wen, Miaoyun, Lian, Zhesi, Huang, Linqiang, Zhu, Senzhi, Hu, Bei, Han, Yongli, Deng, Yiyu, Zeng, Hongke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647722/
https://www.ncbi.nlm.nih.gov/pubmed/29067103
http://dx.doi.org/10.3892/etm.2017.5034
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author Wen, Miaoyun
Lian, Zhesi
Huang, Linqiang
Zhu, Senzhi
Hu, Bei
Han, Yongli
Deng, Yiyu
Zeng, Hongke
author_facet Wen, Miaoyun
Lian, Zhesi
Huang, Linqiang
Zhu, Senzhi
Hu, Bei
Han, Yongli
Deng, Yiyu
Zeng, Hongke
author_sort Wen, Miaoyun
collection PubMed
description The diagnostic value of magnetic resonance spectroscopy (MRS), T2-weighted imaging (T2WI) and serum markers of brain injury in a rat model of sepsis were investigated. Rats were randomly divided into the control group and 6, 12 and 24 h after lipopolysaccharide-injection groups. Brain morphology and metabolism were assessed with T2WI magnetic resonance imaging (MRI) and MRS. Serum and brain tissue samples were then collected to examine the concentrations of neuron-specific enolase (NSE) and S100-β protein. Brain T2WI showed no differences between the groups. N-acetylaspartate/choline (NAA/Cr) ratio measured by MRS showed different degrees of decrease in the sepsis groups, and serum NSE and S100-β concentrations were increased compared with the control group. Apoptosis rates were measured in the right hippocampal area, and there were statistically significant differences between the indicated groups and the control group (p<0.05). The correlation between apoptosis rate and NAA/Cr ratio was closer than that between apoptosis rate and NSE or S100-β (−0.925 vs. 0.434 vs. 0.517, respectively). In conclusion, MRS is a sensitive, non-invasive method to investigate complications of brain injury in septic rats, which may be utilized for the early diagnosis of brain injury caused by sepsis.
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spelling pubmed-56477222017-10-24 Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis Wen, Miaoyun Lian, Zhesi Huang, Linqiang Zhu, Senzhi Hu, Bei Han, Yongli Deng, Yiyu Zeng, Hongke Exp Ther Med Articles The diagnostic value of magnetic resonance spectroscopy (MRS), T2-weighted imaging (T2WI) and serum markers of brain injury in a rat model of sepsis were investigated. Rats were randomly divided into the control group and 6, 12 and 24 h after lipopolysaccharide-injection groups. Brain morphology and metabolism were assessed with T2WI magnetic resonance imaging (MRI) and MRS. Serum and brain tissue samples were then collected to examine the concentrations of neuron-specific enolase (NSE) and S100-β protein. Brain T2WI showed no differences between the groups. N-acetylaspartate/choline (NAA/Cr) ratio measured by MRS showed different degrees of decrease in the sepsis groups, and serum NSE and S100-β concentrations were increased compared with the control group. Apoptosis rates were measured in the right hippocampal area, and there were statistically significant differences between the indicated groups and the control group (p<0.05). The correlation between apoptosis rate and NAA/Cr ratio was closer than that between apoptosis rate and NSE or S100-β (−0.925 vs. 0.434 vs. 0.517, respectively). In conclusion, MRS is a sensitive, non-invasive method to investigate complications of brain injury in septic rats, which may be utilized for the early diagnosis of brain injury caused by sepsis. D.A. Spandidos 2017-11 2017-08-25 /pmc/articles/PMC5647722/ /pubmed/29067103 http://dx.doi.org/10.3892/etm.2017.5034 Text en Copyright: © Wen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wen, Miaoyun
Lian, Zhesi
Huang, Linqiang
Zhu, Senzhi
Hu, Bei
Han, Yongli
Deng, Yiyu
Zeng, Hongke
Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title_full Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title_fullStr Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title_full_unstemmed Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title_short Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
title_sort magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647722/
https://www.ncbi.nlm.nih.gov/pubmed/29067103
http://dx.doi.org/10.3892/etm.2017.5034
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