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Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis
The aim of the present study was to investigate the effect of glucocorticoid intervention on olfactory dysfunction in mice with allergic rhinitis (AR). An AR animal model was established by intraperitoneal injection and intranasal application of ovalbumin to mice. The olfactory function of the mice...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647749/ https://www.ncbi.nlm.nih.gov/pubmed/29075337 http://dx.doi.org/10.3892/etm.2017.5042 |
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author | Wang, Xiaowei Zhu, Yingying Ni, Daofeng Lv, Wei Gao, Zhiqiang Qi, Fang |
author_facet | Wang, Xiaowei Zhu, Yingying Ni, Daofeng Lv, Wei Gao, Zhiqiang Qi, Fang |
author_sort | Wang, Xiaowei |
collection | PubMed |
description | The aim of the present study was to investigate the effect of glucocorticoid intervention on olfactory dysfunction in mice with allergic rhinitis (AR). An AR animal model was established by intraperitoneal injection and intranasal application of ovalbumin to mice. The olfactory function of the mice was evaluated using a buried food test, and morphological changes in the nasal mucosa were determined using hematoxylin and eosin staining. The expression of olfactory marker protein (OMP) in the olfactory mucosa was tested by immunohistochemistry, and was observed on days 7 and 14 after the application of glucocorticoid. The incidence rate of olfactory dysfunction in AR mice was 75.34%, and the olfactory epithelium became thinner in mice with AR compared to the control group. In addition, the expression of OMP in the olfactory epithelium was downregulated in mice with AR compared with the control group. Expression of OMP in the olfactory epithelium was upregulated in the budesonide group A and betamethasone group A compared with the medicine-free group, whereas the expression of OMP in the olfactory epithelium of budesonide group A or betamethasone group A was not significantly different from the control group. Moreover, the expression of OMP in the budesonide group B was similar to budesonide group A, and expression of OMP in betamethasone group B was similar to betamethasone group A. The expression of OMP in olfactory mucosa is downregulated in AR mice with olfactory dysfunction. Following the application of glucocorticoid, the expression of OMP in the olfactory mucosa in mice is upregulated. Moreover, intranasal local glucocorticoid has a low incidence of systemic adverse reactions, and is recommended for the treatment of olfactory dysfunction in AR. |
format | Online Article Text |
id | pubmed-5647749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56477492017-10-26 Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis Wang, Xiaowei Zhu, Yingying Ni, Daofeng Lv, Wei Gao, Zhiqiang Qi, Fang Exp Ther Med Articles The aim of the present study was to investigate the effect of glucocorticoid intervention on olfactory dysfunction in mice with allergic rhinitis (AR). An AR animal model was established by intraperitoneal injection and intranasal application of ovalbumin to mice. The olfactory function of the mice was evaluated using a buried food test, and morphological changes in the nasal mucosa were determined using hematoxylin and eosin staining. The expression of olfactory marker protein (OMP) in the olfactory mucosa was tested by immunohistochemistry, and was observed on days 7 and 14 after the application of glucocorticoid. The incidence rate of olfactory dysfunction in AR mice was 75.34%, and the olfactory epithelium became thinner in mice with AR compared to the control group. In addition, the expression of OMP in the olfactory epithelium was downregulated in mice with AR compared with the control group. Expression of OMP in the olfactory epithelium was upregulated in the budesonide group A and betamethasone group A compared with the medicine-free group, whereas the expression of OMP in the olfactory epithelium of budesonide group A or betamethasone group A was not significantly different from the control group. Moreover, the expression of OMP in the budesonide group B was similar to budesonide group A, and expression of OMP in betamethasone group B was similar to betamethasone group A. The expression of OMP in olfactory mucosa is downregulated in AR mice with olfactory dysfunction. Following the application of glucocorticoid, the expression of OMP in the olfactory mucosa in mice is upregulated. Moreover, intranasal local glucocorticoid has a low incidence of systemic adverse reactions, and is recommended for the treatment of olfactory dysfunction in AR. D.A. Spandidos 2017-11 2017-08-28 /pmc/articles/PMC5647749/ /pubmed/29075337 http://dx.doi.org/10.3892/etm.2017.5042 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Xiaowei Zhu, Yingying Ni, Daofeng Lv, Wei Gao, Zhiqiang Qi, Fang Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title | Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title_full | Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title_fullStr | Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title_full_unstemmed | Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title_short | Intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
title_sort | intranasal application of glucocorticoid alleviates olfactory dysfunction in mice with allergic rhinitis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647749/ https://www.ncbi.nlm.nih.gov/pubmed/29075337 http://dx.doi.org/10.3892/etm.2017.5042 |
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