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Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration
BACKGROUND: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds. METHODS: In this study, the attachments of nerve cells, including Schwann cells,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Dental Society of Anesthsiology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647825/ https://www.ncbi.nlm.nih.gov/pubmed/29090249 http://dx.doi.org/10.17245/jdapm.2017.17.3.191 |
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author | Chung, Joo-Ryun Choi, Jong-Won Fiorellini, Joseph P. Hwang, Kyung-Gyun Park, Chang-Joo |
author_facet | Chung, Joo-Ryun Choi, Jong-Won Fiorellini, Joseph P. Hwang, Kyung-Gyun Park, Chang-Joo |
author_sort | Chung, Joo-Ryun |
collection | PubMed |
description | BACKGROUND: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds. METHODS: In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated. RESULTS: The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF). CONCLUSIONS: Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits. |
format | Online Article Text |
id | pubmed-5647825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Dental Society of Anesthsiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56478252017-10-31 Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration Chung, Joo-Ryun Choi, Jong-Won Fiorellini, Joseph P. Hwang, Kyung-Gyun Park, Chang-Joo J Dent Anesth Pain Med Original Article BACKGROUND: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds. METHODS: In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated. RESULTS: The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF). CONCLUSIONS: Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits. The Korean Dental Society of Anesthsiology 2017-09 2017-09-25 /pmc/articles/PMC5647825/ /pubmed/29090249 http://dx.doi.org/10.17245/jdapm.2017.17.3.191 Text en Copyright © 2017 Journal of Dental Anesthesia and Pain Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Chung, Joo-Ryun Choi, Jong-Won Fiorellini, Joseph P. Hwang, Kyung-Gyun Park, Chang-Joo Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title | Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title_full | Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title_fullStr | Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title_full_unstemmed | Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title_short | Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
title_sort | effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647825/ https://www.ncbi.nlm.nih.gov/pubmed/29090249 http://dx.doi.org/10.17245/jdapm.2017.17.3.191 |
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