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Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy

We performed dye-enhanced imaging of mouse brain microvessels using spectral focusing coherent anti-Stokes Raman scattering (SF-CARS) microscopy. The resonant signals from C-H stretching in forward CARS usually show high background intensity in tissues, which makes CARS imaging of microvessels diffi...

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Autores principales: Lee, Bo-Ram, Joo, Kyung-Il, Choi, Eun Sook, Jahng, Junghoon, Kim, Hyunmin, Kim, Eunjoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648124/
https://www.ncbi.nlm.nih.gov/pubmed/29049299
http://dx.doi.org/10.1371/journal.pone.0185519
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author Lee, Bo-Ram
Joo, Kyung-Il
Choi, Eun Sook
Jahng, Junghoon
Kim, Hyunmin
Kim, Eunjoo
author_facet Lee, Bo-Ram
Joo, Kyung-Il
Choi, Eun Sook
Jahng, Junghoon
Kim, Hyunmin
Kim, Eunjoo
author_sort Lee, Bo-Ram
collection PubMed
description We performed dye-enhanced imaging of mouse brain microvessels using spectral focusing coherent anti-Stokes Raman scattering (SF-CARS) microscopy. The resonant signals from C-H stretching in forward CARS usually show high background intensity in tissues, which makes CARS imaging of microvessels difficult. In this study, epi-detection of back-scattered SF-CARS signals showed a negligible background, but the overall intensity of resonant CARS signals was too low to observe the network of brain microvessels. Therefore, Evans blue (EB) dye was used as contrasting agent to enhance the back-scattered SF-CARS signals. Breakdown of brain microvessels by inducing hemorrhage in a mouse was clearly visualized using backward SF-CARS signals, following intravenous injection of EB. The improved visualization of brain microvessels with EB enhanced the sensitivity of SF-CARS, detecting not only the blood vessels themselves but their integrity as well in the brain vasculature.
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spelling pubmed-56481242017-11-03 Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy Lee, Bo-Ram Joo, Kyung-Il Choi, Eun Sook Jahng, Junghoon Kim, Hyunmin Kim, Eunjoo PLoS One Research Article We performed dye-enhanced imaging of mouse brain microvessels using spectral focusing coherent anti-Stokes Raman scattering (SF-CARS) microscopy. The resonant signals from C-H stretching in forward CARS usually show high background intensity in tissues, which makes CARS imaging of microvessels difficult. In this study, epi-detection of back-scattered SF-CARS signals showed a negligible background, but the overall intensity of resonant CARS signals was too low to observe the network of brain microvessels. Therefore, Evans blue (EB) dye was used as contrasting agent to enhance the back-scattered SF-CARS signals. Breakdown of brain microvessels by inducing hemorrhage in a mouse was clearly visualized using backward SF-CARS signals, following intravenous injection of EB. The improved visualization of brain microvessels with EB enhanced the sensitivity of SF-CARS, detecting not only the blood vessels themselves but their integrity as well in the brain vasculature. Public Library of Science 2017-10-19 /pmc/articles/PMC5648124/ /pubmed/29049299 http://dx.doi.org/10.1371/journal.pone.0185519 Text en © 2017 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Bo-Ram
Joo, Kyung-Il
Choi, Eun Sook
Jahng, Junghoon
Kim, Hyunmin
Kim, Eunjoo
Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title_full Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title_fullStr Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title_full_unstemmed Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title_short Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
title_sort evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-stokes raman scattering microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648124/
https://www.ncbi.nlm.nih.gov/pubmed/29049299
http://dx.doi.org/10.1371/journal.pone.0185519
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