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Electrical impedance myography detects age-related muscle change in mice

Loss of muscle mass and strength represents one of the most significant contributors to impaired function in older adults. Convenient and non-invasive biomarkers are needed that can readily identify and track age-related muscle change. Previous data has suggested electrical impedance myography (EIM)...

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Autores principales: Arnold, W. David, Taylor, Rebecca S., Li, Jia, Nagy, Janice A., Sanchez, Benjamin, Rutkove, Seward B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648130/
https://www.ncbi.nlm.nih.gov/pubmed/29049394
http://dx.doi.org/10.1371/journal.pone.0185614
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author Arnold, W. David
Taylor, Rebecca S.
Li, Jia
Nagy, Janice A.
Sanchez, Benjamin
Rutkove, Seward B.
author_facet Arnold, W. David
Taylor, Rebecca S.
Li, Jia
Nagy, Janice A.
Sanchez, Benjamin
Rutkove, Seward B.
author_sort Arnold, W. David
collection PubMed
description Loss of muscle mass and strength represents one of the most significant contributors to impaired function in older adults. Convenient and non-invasive biomarkers are needed that can readily identify and track age-related muscle change. Previous data has suggested electrical impedance myography (EIM) has the potential to serve in this capacity. In this study we investigated how changes in EIM compared with other standard measures of muscle structure and function in aged compared with young mice. A total of 19 male mice aged approximately 25 months and 19 male mice aged 3 months underwent surface multifrequency EIM of the right gastrocnemius muscle using standard methods. Fore and hind limb grip strength, sciatic compound muscle action potential amplitude, and in-situ force of the gastrocnemius were also measured; after sacrifice, gastrocnemius myofiber size was assessed using standard histology. Spearman correlation coefficients were calculated to investigate the association between EIM and muscle characteristics. EIM in aged animals demonstrated significantly lower 50 kHz impedance phase (p<0.001) and reactance (p<0.01) values as well as reduced multifrequency parameters. In contrast, absolute gastrocnemius muscle mass was no different between young and aged mice (p = 0.58) but was reduced in aged mice after normalization to body mass (p<0.001). Median myofiber size in the aged mice was not different from that of young mice (p = 0.72). Aged mice showed reduced muscle function on the basis of normalized fore limb (p<0.001) and normalized hind limb (p<0.001) grip strength, as well as normalized gastrocnemius twitch (p<0.001) and normalized maximal isometric force (p<0.001). Sciatic compound muscle action potential amplitude was reduced in aged mice (p<0.05). EIM parameters showed good correlation with reduced standard physiological and electrophysiological measures of muscle health. Our study suggests that EIM is sensitive to aged-related muscle change and may represent a convenient and valuable method of quantifying loss of muscle health.
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spelling pubmed-56481302017-11-03 Electrical impedance myography detects age-related muscle change in mice Arnold, W. David Taylor, Rebecca S. Li, Jia Nagy, Janice A. Sanchez, Benjamin Rutkove, Seward B. PLoS One Research Article Loss of muscle mass and strength represents one of the most significant contributors to impaired function in older adults. Convenient and non-invasive biomarkers are needed that can readily identify and track age-related muscle change. Previous data has suggested electrical impedance myography (EIM) has the potential to serve in this capacity. In this study we investigated how changes in EIM compared with other standard measures of muscle structure and function in aged compared with young mice. A total of 19 male mice aged approximately 25 months and 19 male mice aged 3 months underwent surface multifrequency EIM of the right gastrocnemius muscle using standard methods. Fore and hind limb grip strength, sciatic compound muscle action potential amplitude, and in-situ force of the gastrocnemius were also measured; after sacrifice, gastrocnemius myofiber size was assessed using standard histology. Spearman correlation coefficients were calculated to investigate the association between EIM and muscle characteristics. EIM in aged animals demonstrated significantly lower 50 kHz impedance phase (p<0.001) and reactance (p<0.01) values as well as reduced multifrequency parameters. In contrast, absolute gastrocnemius muscle mass was no different between young and aged mice (p = 0.58) but was reduced in aged mice after normalization to body mass (p<0.001). Median myofiber size in the aged mice was not different from that of young mice (p = 0.72). Aged mice showed reduced muscle function on the basis of normalized fore limb (p<0.001) and normalized hind limb (p<0.001) grip strength, as well as normalized gastrocnemius twitch (p<0.001) and normalized maximal isometric force (p<0.001). Sciatic compound muscle action potential amplitude was reduced in aged mice (p<0.05). EIM parameters showed good correlation with reduced standard physiological and electrophysiological measures of muscle health. Our study suggests that EIM is sensitive to aged-related muscle change and may represent a convenient and valuable method of quantifying loss of muscle health. Public Library of Science 2017-10-19 /pmc/articles/PMC5648130/ /pubmed/29049394 http://dx.doi.org/10.1371/journal.pone.0185614 Text en © 2017 Arnold et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Arnold, W. David
Taylor, Rebecca S.
Li, Jia
Nagy, Janice A.
Sanchez, Benjamin
Rutkove, Seward B.
Electrical impedance myography detects age-related muscle change in mice
title Electrical impedance myography detects age-related muscle change in mice
title_full Electrical impedance myography detects age-related muscle change in mice
title_fullStr Electrical impedance myography detects age-related muscle change in mice
title_full_unstemmed Electrical impedance myography detects age-related muscle change in mice
title_short Electrical impedance myography detects age-related muscle change in mice
title_sort electrical impedance myography detects age-related muscle change in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648130/
https://www.ncbi.nlm.nih.gov/pubmed/29049394
http://dx.doi.org/10.1371/journal.pone.0185614
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