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It is time for top-down venomics
The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648493/ https://www.ncbi.nlm.nih.gov/pubmed/29075288 http://dx.doi.org/10.1186/s40409-017-0135-6 |
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author | Melani, Rafael D. Nogueira, Fabio C. S. Domont, Gilberto B. |
author_facet | Melani, Rafael D. Nogueira, Fabio C. S. Domont, Gilberto B. |
author_sort | Melani, Rafael D. |
collection | PubMed |
description | The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics. |
format | Online Article Text |
id | pubmed-5648493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56484932017-10-26 It is time for top-down venomics Melani, Rafael D. Nogueira, Fabio C. S. Domont, Gilberto B. J Venom Anim Toxins Incl Trop Dis Review The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics. BioMed Central 2017-10-18 /pmc/articles/PMC5648493/ /pubmed/29075288 http://dx.doi.org/10.1186/s40409-017-0135-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Melani, Rafael D. Nogueira, Fabio C. S. Domont, Gilberto B. It is time for top-down venomics |
title | It is time for top-down venomics |
title_full | It is time for top-down venomics |
title_fullStr | It is time for top-down venomics |
title_full_unstemmed | It is time for top-down venomics |
title_short | It is time for top-down venomics |
title_sort | it is time for top-down venomics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648493/ https://www.ncbi.nlm.nih.gov/pubmed/29075288 http://dx.doi.org/10.1186/s40409-017-0135-6 |
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