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It is time for top-down venomics

The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (...

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Autores principales: Melani, Rafael D., Nogueira, Fabio C. S., Domont, Gilberto B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648493/
https://www.ncbi.nlm.nih.gov/pubmed/29075288
http://dx.doi.org/10.1186/s40409-017-0135-6
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author Melani, Rafael D.
Nogueira, Fabio C. S.
Domont, Gilberto B.
author_facet Melani, Rafael D.
Nogueira, Fabio C. S.
Domont, Gilberto B.
author_sort Melani, Rafael D.
collection PubMed
description The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics.
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spelling pubmed-56484932017-10-26 It is time for top-down venomics Melani, Rafael D. Nogueira, Fabio C. S. Domont, Gilberto B. J Venom Anim Toxins Incl Trop Dis Review The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics. BioMed Central 2017-10-18 /pmc/articles/PMC5648493/ /pubmed/29075288 http://dx.doi.org/10.1186/s40409-017-0135-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Melani, Rafael D.
Nogueira, Fabio C. S.
Domont, Gilberto B.
It is time for top-down venomics
title It is time for top-down venomics
title_full It is time for top-down venomics
title_fullStr It is time for top-down venomics
title_full_unstemmed It is time for top-down venomics
title_short It is time for top-down venomics
title_sort it is time for top-down venomics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648493/
https://www.ncbi.nlm.nih.gov/pubmed/29075288
http://dx.doi.org/10.1186/s40409-017-0135-6
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